Gonadorelin-Induced Testosterone Release: A Biological Assay for Quality Assurance of Gonadorelin in Veterinary Medicine
| Autor: | R. N. Summers, D.J. Kesler, T. L. Steckler, C. A. Peterson |
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| Rok vydání: | 1999 |
| Předmět: |
Male
Quality Control medicine.medical_specialty Time Factors Assurance qualite Pharmaceutical Science Pharmacology Gonadoréline Biological effect Gonadotropin-Releasing Hormone chemistry.chemical_compound Pregnenediones Drug Discovery medicine Animals Bioassay Testosterone Drug Implants business.industry Organic Chemistry Testosterone (patch) Norgestomet Surgery chemistry Gonadorelina Biological Assay Cattle Progestins business |
| Zdroj: | Drug Development and Industrial Pharmacy. 25:315-321 |
| ISSN: | 1520-5762 0363-9045 |
| DOI: | 10.1081/ddc-100102176 |
| Popis: | Two experiments were conducted with bulls administered norgestomet and gonadorelin to determine if the gonadorelin-induced release of testosterone could be developed into a biological assay for quality assurance of gonadorelin. Implants containing norgestomet (0 to 36 mg) reduced the episodic release (r = -.81; P.05) and mean concentrations of testosterone (r = -.82; P.05). Gonadoreline-induced testosterone release increased (r = .99; P.05) with increasing dosage of gonadorelin (up to 5 micrograms) in norgestomet-implanted bulls (36 mg). Maximal testosterone was released (sixfold increase) with 5 to 40 micrograms of gonadorelin. In summary, the gonadorelin-induced testosterone release in bulls administered a synthetic progestin is a sensitive (0.008 microgram per kg body weight for 5 micrograms of gonadorelin) biological assay with a rapid turnaround time for the confirmation of gonadorelin potency. Based on a per-kg-body-weight basis, the norgestomet-treated bull is the most sensitive biological assay model. |
| Databáze: | OpenAIRE |
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