An Essential Role for Bacterial Nitric Oxide Synthase in Staphylococcus aureus Electron Transfer and Colonisation
Autor: | Stephen J. Libby, Smirla Ramos-Montañez, Jasmine M. Pando, Daniel V. Tadeo, Erin N. Strom, Traci L. Kinkel, Ferric C. Fang |
---|---|
Jazyk: | angličtina |
Rok vydání: | 2016 |
Předmět: |
0301 basic medicine
Microbiology (medical) Staphylococcus aureus Cytochrome Immunology Human pathogen medicine.disease_cause Applied Microbiology and Biotechnology Microbiology Article Nitric oxide Electron Transport Electron Transport Complex IV 03 medical and health sciences chemistry.chemical_compound Genetics medicine polycyclic compounds Animals chemistry.chemical_classification biology ATP synthase digestive oral and skin physiology Cell Biology biology.organism_classification Aerobiosis Nitric oxide synthase Mice Inbred C57BL 030104 developmental biology Enzyme chemistry biology.protein Nasal Cavity Nitric Oxide Synthase Bacteria |
Popis: | Nitric oxide (NO•) is a ubiquitous molecular mediator in biology. Many signalling actions of NO• generated by mammalian NO• synthase (NOS) result from targeting of the haem moiety of soluble guanylate cyclase. Some pathogenic and environmental bacteria also produce a NOS that is evolutionary related to the mammalian enzymes, but a bacterial haem-containing receptor for endogenous enzymatically generated NO• has not been identified previously. Here, we show that NOS of the human pathogen Staphylococcus aureus, in concert with an NO•-metabolizing flavohaemoprotein, regulates electron transfer by targeting haem-containing cytochrome oxidases under microaerobic conditions to maintain membrane bioenergetics. This process is essential for staphylococcal nasal colonization and resistance to the membrane-targeting antibiotic daptomycin and demonstrates the conservation of NOS-derived NO•-haem receptor signalling between bacteria and mammals. |
Databáze: | OpenAIRE |
Externí odkaz: |