Elucidating constraints for differentiation of major human Klebsiella pneumoniae clones using MALDI-TOF MS
| Autor: | Clara Sousa, Carla Rodrigues, T. M. Coque, João A. Lopes, Ângela Novais, Rafael Cantón, Helena Ramos, Luísa Peixe |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Microbiology (medical) biology Portugal Klebsiella pneumoniae 030106 microbiology Bacterial typing General Medicine Sequence types biology.organism_classification Microbiology Bacterial Typing Techniques Klebsiella Infections Multiple drug resistance Discriminatory power 03 medical and health sciences Matrix-assisted laser desorption/ionization Infectious Diseases Ribosomal protein Spain Spectrometry Mass Matrix-Assisted Laser Desorption-Ionization Humans Typing |
| Zdroj: | European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 36(2) |
| ISSN: | 1435-4373 |
| Popis: | The establishment of matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF MS) in routine microbial identification boosted many developments towards high-throughput applications, including bacterial typing. However, results are still controversial for different bacterial species. We aim to evaluate the suitability of MALDI-TOF MS for typing clinically relevant multidrug resistant (MDR) Klebsiella pneumoniae subsp. pneumoniae clones using routine conditions and a previously validated chemometric analysis workflow. Mass spectra of 83 K. pneumoniae clinical isolates representing major human MDR clones [11 sequence types (STs), 22 PFGE-types] recovered in Portugal and Spain during outbreaks and non-outbreak situations (2003-2012) were obtained from cell extracts (CE) and intact cells (IC), and analysed with different chemometric tools. We observed a highly consistent peak pattern among isolates from different clones either with CE or IC, suggesting a high degree of conservation of biomolecules analysed (a large part corresponding to ribosomal proteins). Moreover, the low degree of agreement between MALDI-TOF MS and other methods (from 34.9 % to 43.4 % of correct assignments for CE and from 40.8 % to 70.1 % for IC) corroborates the low discriminatory potential of the technique at infraspecies level. Our results suggest a low discriminatory power of MALDI-TOF MS for clinically relevant MDR K. pneumoniae clones and highlight the need of developing tools for high-resolution typing in this species. |
| Databáze: | OpenAIRE |
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