Widespread distribution of tetracycline resistance genes in a confined animal feeding facility
| Autor: | Maryruth S. Stine, O. Colin Stine, Judith A. Johnson, Kellie Perry, J. Glenn Morris, S. Qaiyumi, Ananda Keefer-Norris, Justine Tigno |
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| Rok vydání: | 2006 |
| Předmět: |
Microbiology (medical)
DNA Bacterial Tetracycline Sus scrofa medicine.disease_cause Feces Antibiotic resistance Sequence Homology Nucleic Acid medicine Environmental Microbiology Escherichia coli Animals Pharmacology (medical) Animal Husbandry Gene Alleles Antibacterial agent Genetics biology Bacteria Base Sequence Tetracycline Resistance General Medicine biology.organism_classification 16S ribosomal RNA Animal Feed genomic DNA Infectious Diseases Genes Bacterial Food Microbiology Enterococcus medicine.drug |
| Zdroj: | International journal of antimicrobial agents. 29(3) |
| ISSN: | 0924-8579 |
| Popis: | We sought to determine the distribution of resistance and the tetracycline resistance genes among bacteria isolated from a swine confined animal feeding facility where tetracycline-containing feed had been in use for over 20 years. Samples collected from feed, hogs, hog houses, waste lagoon, soil, surface water and well water were screened for the presence of (a) resistant Escherichia coli and enterococci and (b) tetracycline-resistant strains of all species. Genomic DNA was extracted from the latter strain collection and fragments from 16S rDNA and ten tetracycline resistance genes (tetA, tetB, tetC, tetE, tetH, tetL, tetM, tetS, tetT and rumB) were polymerase chain reaction-amplified and a partial nucleotide sequence was obtained. In this environment, 77% of E. coli and 68% of enterococci isolated were tetracycline resistant. Tetracycline resistance was found in 26 different bacterial genera and in 60 species. Single resistance gene alleles (as defined by nucleotide sequence) were present in multiple species. There was evidence of gene recombination and multiple different tetracycline resistance genes were present in single bacterial isolates. These data provide further evidence for the widespread distribution of resistance genes in microbial populations in settings in which there is ongoing subtherapeutic antimicrobial use. |
| Databáze: | OpenAIRE |
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