Integrated Amplification Microarrays for Infectious Disease Diagnostics
| Autor: | Lexi Bryant, Sara B. Griesemer, Cynthia Zimmerman, Jennifer Parker, Christopher Knickerbocker, Alexander Kukhtin, Kirsten St. George, Christopher G. Cooney, Rui Gu, Darrell P. Chandler |
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| Rok vydání: | 2012 |
| Předmět: |
Microarray
Microfluidics microfluidics RT-PCR Biomedical Engineering Bioengineering Review Computational biology Biology asymmetric PCR Bioinformatics Biochemistry Multiplexing integrated microarrays lcsh:Biochemistry 03 medical and health sciences reverse transcriptase diagnostics lcsh:QD415-436 Multiplex 030304 developmental biology gel element arrays 0303 health sciences 030306 microbiology Amplicon multiplex ComputingMethodologies_PATTERNRECOGNITION Workflow Infectious disease (medical specialty) DNA microarray Biotechnology |
| Zdroj: | Microarrays Microarrays, Vol 1, Iss 3, Pp 107-124 (2012) |
| ISSN: | 2076-3905 |
| Popis: | This overview describes microarray-based tests that combine solution-phase amplification chemistry and microarray hybridization within a single microfluidic chamber. The integrated biochemical approach improves microarray workflow for diagnostic applications by reducing the number of steps and minimizing the potential for sample or amplicon cross-contamination. Examples described herein illustrate a basic, integrated approach for DNA and RNA genomes, and a simple consumable architecture for incorporating wash steps while retaining an entirely closed system. It is anticipated that integrated microarray biochemistry will provide an opportunity to significantly reduce the complexity and cost of microarray consumables, equipment, and workflow, which in turn will enable a broader spectrum of users to exploit the intrinsic multiplexing power of microarrays for infectious disease diagnostics. |
| Databáze: | OpenAIRE |
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