Use of a Rapid Test for Diagnosis of Dengue during Suspected Dengue Outbreaks in Resource-Limited Regions
| Autor: | Elizabeth Hunsperger, Taina Naivalu, Maria Marfel, W. Thane Hancock, Paul Lalita, Aalisha Sahu Khan, Tyler M. Sharp, Harold S. Margolis, Kay M. Tomashek, Kini Tikomaidraubuta, Yolanda Rebello Cardoso |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Adult
Male Microbiology (medical) Serotype medicine.medical_specialty Time Factors Adolescent viruses 030231 tropical medicine Suspected dengue Viral Nonstructural Proteins Dengue virus Antibodies Viral medicine.disease_cause Sensitivity and Specificity Disease Outbreaks Dengue fever Dengue Young Adult 03 medical and health sciences 0302 clinical medicine Internal medicine parasitic diseases medicine Humans Prospective Studies 030212 general & internal medicine Immunoassays Child Aged Aged 80 and over Immunoassay Rapid diagnostic test biology Diagnostic Tests Routine business.industry Infant Newborn Infant virus diseases Outbreak Middle Aged medicine.disease Immunoglobulin M Specimen collection Child Preschool biology.protein Female business |
| Zdroj: | Journal of Clinical Microbiology. 54:2090-2095 |
| ISSN: | 1098-660X 0095-1137 |
| DOI: | 10.1128/jcm.00521-16 |
| Popis: | Dengue is major public health problem, globally. Timely verification of suspected dengue outbreaks allows for public health response, leading to the initiation of appropriate clinical care. Because the clinical presentation of dengue is nonspecific, dengue diagnosis would benefit from a sensitive rapid diagnostic test (RDT). We evaluated the diagnostic performance of an RDT that detects dengue virus (DENV) nonstructural protein 1 (NS1) and anti-DENV IgM during suspected acute febrile illness (AFI) outbreaks in four countries. Real-time reverse transcription-PCR and anti-DENV IgM enzyme-linked immunosorbent assay were used to verify RDT results. Anti-DENV IgM RDT sensitivity and specificity ranged from 55.3 to 91.7% and 85.3 to 98.5%, respectively, and NS1 sensitivity and specificity ranged from 49.7 to 92.9% and 22.2 to 89.0%, respectively. Sensitivity varied by timing of specimen collection and DENV serotype. Combined test results moderately improved the sensitivity. The use of RDTs identified dengue as the cause of AFI outbreaks where reference diagnostic testing was limited or unavailable. |
| Databáze: | OpenAIRE |
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