The Arabidopsis mTERF‐repeat MDA1 protein plays a dual function in transcription and stabilization of specific chloroplast transcripts within the psbE and ndhH operons
| Autor: | Rabea Ghandour, Aude Zimmerman, Louis Valentin Méteignier, Jörg Meurer, Abdelmalek Alioua, Karin Meierhoff, Johana Chicher, Reimo Zoschke, Nicolas Baumberger, Kamel Hammani |
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| Přispěvatelé: | Institut de biologie moléculaire des plantes (IBMP), Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA), Institut de génétique et biologie moléculaire et cellulaire (IGBMC), Université Louis Pasteur - Strasbourg I-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE20-0007,plastRNP,Étude du rôle régulateur des protéines de liaison à l'ARN dans la réponse génétique du chloroplaste et l'adaptation des plantes aux variations développementales et environnementales(2016), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Louis Pasteur - Strasbourg I |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Chloroplasts Physiology Arabidopsis Plant Science Biology 01 natural sciences Chloroplast Proteins 03 medical and health sciences chemistry.chemical_compound Gene Expression Regulation Plant Transcription (biology) RNA polymerase Operon Gene expression [SDV.BV]Life Sciences [q-bio]/Vegetal Biology Gene family Sciences du Vivant [q-bio]/Biologie végétale plastid Gene ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Full Paper Arabidopsis Proteins Research food and beverages RNA DNA MRNA stabilization Full Papers Cell biology helical repeat protein chemistry Mutation gene expression mTERF Transcription Factors 010606 plant biology & botany |
| Zdroj: | New Phytologist New Phytologist, Wiley, 2020, ⟨10.1111/nph.16625⟩ The New Phytologist |
| ISSN: | 1469-8137 0028-646X |
| DOI: | 10.1111/nph.16625 |
| Popis: | Summary The mTERF gene family encodes for nucleic acid binding proteins that are predicted to regulate organellar gene expression in eukaryotes. Despite the implication of this gene family in plant development and response to abiotic stresses, a precise molecular function was assigned to only a handful number of its c. 30 members in plants.Using a reverse genetics approach in Arabidopsis thaliana and combining molecular and biochemical techniques, we revealed new functions for the chloroplast mTERF protein, MDA1.We demonstrated that MDA1 associates in vivo with components of the plastid‐encoded RNA polymerase and transcriptional active chromosome complexes. MDA1 protein binds in vivo and in vitro with specificity to 27‐bp DNA sequences near the 5′‐end of psbE and ndhA chloroplast genes to stimulate their transcription, and additionally promotes the stabilization of the 5′‐ends of processed psbE and ndhA messenger (m)RNAs. Finally, we provided evidence that MDA1 function in gene transcription likely coordinates RNA folding and the action of chloroplast RNA‐binding proteins on mRNA stabilization.Our results provide examples for the unexpected implication of DNA binding proteins and gene transcription in the regulation of mRNA stability in chloroplasts, blurring the boundaries between DNA and RNA metabolism in this organelle. |
| Databáze: | OpenAIRE |
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