Repeated ER-endosome contacts promote endosome translocation and neurite outgrowth
| Autor: | Marina Vietri, Eva Maria Wenzel, Veronica Nisi, Hallvard Lauritz Olsvik, Nina Pedersen, Kay Oliver Schink, Sebastian W. Schultz, Harald Stenmark, Andreas Brech, Terje Johansen, Cecilia Bucci, Camilla Raiborg |
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| Přispěvatelé: | Raiborg, Camilla, Wenzel, Eva M, Pedersen, Nina M, Olsvik, Hallvard, Schink, Kay O, Schultz, Sebastian W, Vietri, Marina, Nisi, Veronica, Bucci, Cecilia, Brech, Andrea, Johansen, Terje, Stenmark, Harald |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Neurite
Endosome Transcription Factor DNA-Binding Protein Endocytic cycle Dynein Microtubule Biology Endoplasmic Reticulum HeLa Cell Cell Line Cell membrane Vesicular Transport Protein medicine Multidisciplinary Animal Phosphatidylinositol Phosphate Endoplasmic reticulum Cell Membrane Binding Site Biological Transport Kinesin Synaptotagmin Cell biology medicine.anatomical_structure rab GTP-Binding Proteins Rat Human |
| Popis: | The main organelles of the secretory and endocytic pathways--the endoplasmic reticulum (ER) and endosomes, respectively--are connected through contact sites whose numbers increase as endosomes mature. One function of such sites is to enable dephosphorylation of the cytosolic tails of endosomal signalling receptors by an ER-associated phosphatase, whereas others serve to negatively control the association of endosomes with the minus-end-directed microtubule motor dynein or mediate endosome fission. Cholesterol transfer and Ca(2+) exchange have been proposed as additional functions of such sites. However, the compositions, activities and regulations of ER-endosome contact sites remain incompletely understood. Here we show in human and rat cell lines that protrudin, an ER protein that promotes protrusion and neurite outgrowth, forms contact sites with late endosomes (LEs) via coincident detection of the small GTPase RAB7 and phosphatidylinositol 3-phosphate (PtdIns(3)P). These contact sites mediate transfer of the microtubule motor kinesin 1 from protrudin to the motor adaptor FYCO1 on LEs. Repeated LE-ER contacts promote microtubule-dependent translocation of LEs to the cell periphery and subsequent synaptotagmin-VII-dependent fusion with the plasma membrane. Such fusion induces outgrowth of protrusions and neurites, which requires the abilities of protrudin and FYCO1 to interact with LEs and kinesin 1. Thus, protrudin-containing ER-LE contact sites are platforms for kinesin-1 loading onto LEs, and kinesin-1-mediated translocation of LEs to the plasma membrane, fuelled by repeated ER contacts, promotes protrusion and neurite outgrowth. |
| Databáze: | OpenAIRE |
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