Assessing Chronological Aging in Saccharomyces cerevisiae
Autor: | Mario G. Mirisola, Min Wei, Jia Hu, Valter D. Longo |
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Přispěvatelé: | Galluzzi, L, Vitale, I, Kepp, O, Kroemer, G, Hu, J, Wei, M, Mirisola, MG, Longo, VD |
Rok vydání: | 2012 |
Předmět: |
Cell Nucleus
Genome instability Genetics Mutation rate education.field_of_study Time Factors biology ved/biology Saccharomyces cerevisiae ved/biology.organism_classification_rank.species Population Fungal genetics Water biology.organism_classification Article Genomic Instability Yeast Culture Media yeast genetics aging chronological aging Mutation Rate DNA Fungal Model organism education Gene |
Zdroj: | Methods in Molecular Biology ISBN: 9781627032384 |
Popis: | Saccharomyces cerevisiae is one of the most studied model organisms for the identification of genes and mechanisms that affect aging. The chronological lifespan (CLS) assay, which monitors the survival of a non-dividing population, is one of the two methods to study aging in yeast. To eliminate potential artifacts and identify genes and signaling pathways that may also affect aging in higher eukaryotes, it is important to determine CLS by multiple methods. Here, we describe these methods as well as the assays to study macromolecular damage during aging in yeast, with a focus on genomic instability. |
Databáze: | OpenAIRE |
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