Trigeminal neuropathic pain is alleviated by inhibition of Cav3.3 T-type calcium channels in mice
| Autor: | Leos Cmarko, Sascha R.A. Alles, Marena A. Montera, Aleyah Goins, Karin N. Westlund, Norbert Weiss |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Biophysics Pharmacology Neuropathic pain Biochemistry Calcium Channels T-Type Mice 03 medical and health sciences 0302 clinical medicine trigeminal nerve injury calcium channels Ganglia Spinal therapeutics Animals Medicine Voltage-dependent calcium channel business.industry Brief Report Calcium channel T-type calcium channel Up-Regulation Cav3.3 030104 developmental biology Hyperalgesia Trigeminal nerve injury business 030217 neurology & neurosurgery |
| Zdroj: | Channels article-version (VoR) Version of Record |
| ISSN: | 1933-6969 1933-6950 |
| Popis: | In this brief report, we demonstrate that the Cav3.3 T-type voltage-gated calcium channel subtype is involved in our FRICT-ION model of chronic trigeminal neuropathic pain. We first showed that the Cacna1i gene encoding Cav3.3 is significantly upregulated in whole trigeminal ganglia of FRICT-ION mice compared to controls at week 10 post-injury. We confirmed protein upregulation of Cav3.3 compared to controls using Western blot analysis of whole trigeminal ganglia tissues. Finally, we demonstrated that intraperitoneal injection of a selective TAT-based Cav3.3 blocking peptide in FRICT-ION mice significantly reduces Cav3.3 protein expression at the peak anti-allodynic effect (4 hrs post-injection) of the attenuated neuropathic pain behavior. We also suggest that blockade of Cav3.3 may be more effective in attenuating trigeminal neuropathic pain in female than male FRICT-ION mice. Therefore, blocking or attenuating Cav3.3 function may be an effective strategy for the treatment of trigeminal neuropathic pain. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|