Direct ROS Scavenging Activity of CueP from Salmonella enterica serovar Typhimurium
| Autor: | Si-Hyeon Um, Nam-Chul Ha, Ji-Hyun Yeom, Inseong Jo, Yong-Hak Kim, Bo-Young Yoon, Kangseok Lee, Jin-Sik Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Salmonella typhimurium
Salmonella chemistry.chemical_element medicine.disease_cause Microbiology Cell Line Fenton reaction Mice Bacterial Proteins CueP Phagosomes medicine copper resistance Escherichia coli Animals Molecular Biology Histidine Research Articles Phagosome biology Macrophages Cell Biology General Medicine Periplasmic space Hydrogen Peroxide biology.organism_classification Copper Biochemistry chemistry Salmonella enterica Salmonella Infections Carrier Proteins Reactive Oxygen Species Cysteine |
| Zdroj: | Molecules and Cells |
| ISSN: | 0219-1032 1016-8478 |
| Popis: | Salmonella enterica serovar Typhimurium (S. Typhimurium) is an intracellular pathogen that has evolved to survive in the phagosome of macrophages. The periplasmic copper-binding protein CueP was initially known to confer copper resistance to S. Typhimurium. Crystal structure and biochemical studies on CueP revealed a putative copper binding site surrounded by the conserved cysteine and histidine residues. A recent study reported that CueP supplies copper ions to periplasmic Cu, Zn-superoxide dismutase (SodCII) at a low copper concentration and thus enables the sustained SodCII activity in the periplasm. In this study, we investigated the role of CueP in copper resistance at a high copper concentration. We observed that the survival of a cueP-deleted strain of Salmonella in macrophage phagosome was significantly reduced. Subsequent biochemical experiments revealed that CueP specifically mediates the reduction of copper ion using electrons released during the formation of the disulfide bond. We observed that the copper ion-mediated Fenton reaction in the presence of hydrogen peroxide was blocked by CueP. This study provides insight into how CueP confers copper resistance to S. Typhimurium in copper-rich environments such as the phagosome of macrophages. |
| Databáze: | OpenAIRE |
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