Study of nonspecific cation channel coupled to P2z purinergic receptors using an acid load technique
| Autor: | Jean-Paul Dehaye, Mourad Metioui, Eduardo Alzola, M Lachish, Elie Kabre, Arie Moran, Naima Chaib, Aida Marino, Katrina Grosfils |
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| Rok vydání: | 1996 |
| Předmět: |
Male
Physiology Intracellular pH Submandibular Gland Ion Channels Amiloride chemistry.chemical_compound Cations Muscarinic acetylcholine receptor Animals Ammonium Rats Wistar Receptor Fluorescent Dyes Ion Transport Receptors Purinergic P2 Purinergic receptor Depolarization Cell Biology Hydrogen-Ion Concentration Fluoresceins Rats chemistry Biochemistry Sodium propionate Biophysics Intracellular |
| Zdroj: | The American journal of physiology. 271(6 Pt 1) |
| ISSN: | 0002-9513 |
| Popis: | The intracellular pH (pHi) of rat submandibular cells was measured by 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein (BCECF). The cells recovered from ammonium (30 mM) prepulse to their resting pHi within 10 min. Ethylisopropylamiloride (EIPA), an inhibitor of the Na+/H+ exchanger, slows the rate of pHi recovery. ATP (1 mM), in the presence of EIPA, increases the rate of recovery 3.7-fold in the absence or presence of ethylene glycol-bis(beta-aminoethyl ether)-N,N,N',N'-tetraacetic acid. The recovery was blocked by the addition of 5 mM Mg2+ or 10 microM Coomassie blue. The response was elicited by 2'- and 3'-O-(4-benzoylbenzoyl)-adenosine 5'-triphosphate but not by ADP, UTP, adenyl (beta-gamma-methylene)-diphosphonate, 2-methylthioadenosine 5'-triphosphate, or muscarinic or beta-adrenergic agonists. The purinergic response was also observed when the cells were acidified by sodium propionate and could not be mimicked by the depolarization of the plasma membrane. Aluminum fluoride did not reproduce the response to ATP, suggesting that the observed response does not involve a high-molecular-weight GTP-binding protein. It is concluded that the activation of P2z receptors, probably by the opening of nonspecific cation channels, increases the permeability to protons in rat submandibular glands. |
| Databáze: | OpenAIRE |
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