Dendritic Cell Activation Prevents MHC Class II Ubiquitination and Promotes MHC Class II Survival Regardless of the Activation Stimulus
| Autor: | Even Walseng, Alan Sher, Romina S. Goldszmid, Karis A. Weih, Kazuyuki Furuta, Paul A. Roche |
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| Rok vydání: | 2010 |
| Předmět: |
Surface Properties
Immunology chemical and pharmacologic phenomena Stimulation Cell Separation Ligands Major histocompatibility complex Biochemistry Monocytes Cell membrane Mice Downregulation and upregulation medicine Animals Humans Receptor Molecular Biology Cells Cultured MHC class II biology Intracellular parasite Cell Membrane Toll-Like Receptors Histocompatibility Antigens Class II Dendritic Cells Cell Biology Dendritic cell Flow Cytometry Molecular biology Up-Regulation Cell biology medicine.anatomical_structure biology.protein Toxoplasma |
| Zdroj: | Journal of Biological Chemistry. 285:41749-41754 |
| ISSN: | 0021-9258 |
| Popis: | The expression of MHC class II (MHC-II) on the surface of antigen-presenting cells, such as dendritic cells (DCs), is tightly regulated during cellular activation. Many cells, including DCs, are activated following stimulation of innate Toll-like receptors (TLRs) by products of microorganisms. In the resting (immature) state, MHC-II is ubiquitinated in immature DCs and is rapidly degraded; however, after activation of these cells with MyD88-dependent TLR ligands, MHC-II ubiquitination is blocked, and MHC-II survival is prolonged. We now show that DC activation using MyD88-dependent TLR ligands, MyD88-independent TLR ligands, and even infection with the intracellular parasite Toxoplasma gondii leads to identical changes in MHC-II expression, ubiquitination, and surface stability, revealing a conserved role for enhanced MHC-II stability after DC activation by different stimuli. |
| Databáze: | OpenAIRE |
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