Synthesis of 9-(?-d-arabinofuranosyl)guanine using whole cells of Escherichia coli
| Autor: | Vladimir N. Barai, A. I. Zinchenko, S. B. Bokut, E. I. Kvasyuk, Igor A. Mikhailopulo |
|---|---|
| Rok vydání: | 1990 |
| Předmět: |
Guanine
Stereochemistry Guanosine Nucleoside Deaminases medicine.disease_cause Applied Microbiology and Biotechnology Cytosine Deaminase chemistry.chemical_compound Bacterial Proteins Biosynthesis Escherichia coli medicine Uridine Phosphorylase biology Cytarabine food and beverages General Medicine biochemical phenomena metabolism and nutrition biology.organism_classification Phosphate Enterobacteriaceae Guanine Nucleotides carbohydrates (lipids) Purine-Nucleoside Phosphorylase chemistry Glutaral Yield (chemistry) Arabinonucleosides Cytosine Biotechnology |
| Zdroj: | Applied Microbiology and Biotechnology. 32:658-661 |
| ISSN: | 1432-0614 0175-7598 |
| DOI: | 10.1007/bf00164735 |
| Popis: | Synthesis of 9-(beta-D-arabinofuranosyl)guanine (ara-G) from 1-(beta-D-arabinofuranosyl)cytosine (ara-C) and guanine, guanosine or 2'-deoxyguanosine (dG) by glutaraldehyde-treated Escherichia coli BM-11 cells is described. It is shown that the concentration of phosphate ions, molar ratio of substrates and pH of the reaction medium are factors affecting product yield. Under optimum conditions ara-G was produced in the reaction mixture in a yield of 63%-65% based on dG as the best source of guanine base. The yield of isolated ara-G was 48%-53%. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full text from SpringerLink