Employment of methylene blue irradiated with laser light source in photodynamic inactivation of biofilm formed by Candida albicans strain resistant to fluconazole
Autor: | Lucia Černáková, Stanislava Dižová, Helena Bujdáková |
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Rok vydání: | 2016 |
Předmět: |
inorganic chemicals
0301 basic medicine 030103 biophysics Antifungal Agents Hypha 030106 microbiology Hyphae Microbiology 03 medical and health sciences chemistry.chemical_compound Drug Resistance Fungal Candida albicans Humans Irradiation Fluconazole Microbial Viability Photosensitizing Agents biology Strain (chemistry) Chemistry Lasers Biofilm General Medicine biology.organism_classification Yeast Corpus albicans nervous system diseases body regions Methylene Blue Infectious Diseases Biofilms Biophysics Methylene blue |
Zdroj: | Medical mycology. 55(7) |
ISSN: | 1460-2709 |
Popis: | A promising approach for the eradication of biofilm formed by the yeast Candida albicans seems to be photodynamic inactivation (PDI). This work presents a use of methylene blue (MB, 1 mM) irradiated with a red laser (output power 190 mW/cm2, wavelength 660 nm) for the eradication of a biofilm formed by the fluconazole-resistant (FLC-resistant) strain C. albicans CY 1123 compared to the standard strain C. albicans SC5314. The periods of irradiation corresponded to the fluence of 15, 23 and 57 J/cm2. Effectiveness of PDI was evident with following percentage of survived biofilm cells: 24.57, 23.46, and 22.29% for SC5314 and 40.28, 17.91, and 5.89% for CY 1123, respectively, compared to the samples without irradiation. Light and confocal laser scanning microscopy confirmed the effectiveness of PDI. However, the morphological form of C. albicans seems to play an important role as well, since prolonged duration of irradiation did not increase efficiency of PDI on C. albicans SC5314. An experiment with the yeast-to-hyphae transition revealed that the FLC-resistant strain expressed a markedly reduced capacity to form hyphae compared to SC5314. We summarized that PDI was effective on biofilm formed by the FLC-resistant strain, but resistance most likely did not play significant role in PDI. Additionally, we observed differences in susceptibility to PDI between biofilms composed of the mycelia and only of the yeasts, and finally, the employment of a laser in PDI enabled a decreasing period of irradiation while maintaining the high effectiveness of PDI. |
Databáze: | OpenAIRE |
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