First molecular characterization of Blastocystis subtypes from animals and animal-keepers stool in Algeria
| Autor: | Fadila Tazerouti, Khaled Harhoura, Miriem Aissi, Tahar Kernif, Amina Boutellis, Rezak Drali |
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| Rok vydání: | 2020 |
| Předmět: |
Immunology
Blastocystis Infections Biology Microbiology DNA Ribosomal Feces Immunology and Allergy Animals Gene Ribosomal DNA Phylogeny Blastocystis General Veterinary Phylogenetic tree Haplotype Genetic Variation General Medicine DNA Protozoan biology.organism_classification Infectious Diseases Animal groups Algeria Small subunit Blastocystis sp |
| Zdroj: | Comparative immunology, microbiology and infectious diseases. 78 |
| ISSN: | 1878-1667 |
| Popis: | Blastocystis sp. is one of the most common enteric parasites found in humans and many non-human hosts. It is an anaerobic protozoan that belongs to the group of Stramenopiles. Based on phylogenetic analysis of ribosomal DNA genes, at least 17 subtypes (ST1-ST17) are described. The aim of this study was to identify and characterize Blastocystis sp. in stool samples from various animal groups and animal-keepers. Overall, 29/70 (41.43%) animals and 7/60 (11.66%) humans sampled were positive for Blastocystis sp. using microscopy. The sequencing of the partial 18S small subunit ribosomal DNA gene (SSU rDNA) revealed the presence of five haplotypes corresponding to ST2 and ST3 in humans, and ST2, ST3, ST7, and ST10 in animals. This is the first report of Blastocystis subtypes in animals in Algeria. |
| Databáze: | OpenAIRE |
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