Forms of Lactoferrin: Their Antibacterial Effect on Enterotoxigenic Escherichia coli
| Autor: | David A. Dionysius, Joanne M. Milne, Paul A. Grieve |
|---|---|
| Rok vydání: | 1993 |
| Předmět: |
Siderophore
Bacterial growth medicine.disease_cause Microbiology Enterotoxins chemistry.chemical_compound fluids and secretions Enterotoxigenic Escherichia coli Escherichia coli Genetics medicine Animals Antibacterial agent biology Lactoferrin food and beverages Kinetics stomatognathic diseases chemistry Biochemistry biology.protein Cattle Muramidase Animal Science and Zoology Lysozyme Apoproteins Antibacterial activity Food Science |
| Zdroj: | Journal of Dairy Science. 76:2597-2606 |
| ISSN: | 0022-0302 |
| DOI: | 10.3168/jds.s0022-0302(93)77594-3 |
| Popis: | The antibacterial effects of various forms of lactoferrin on enterotoxigenic strains of Escherichia coli were tested in vitro using a microassay for bacterial growth. Native and apo-lactoferrin exhibited variable activity against 19 strains, whereas holo-lactoferrin had no effect. At a concentration of .2 mg/ml of apo-lactoferrin, strains could be distinguished as either sensitive or resistant to inhibition. Zinc-saturated lactoferrin was as inhibitory as apo-lactoferrin when sensitive and resistant strains were tested over the concentration range .04 to 1.0 mg/ml of lactoferrin. A bactericidal effect was observed for native, apo-, and Zn-saturated lactoferrin against some sensitive strains. The antibacterial activity of apo-lactoferrin depended on bacterial inoculum size and was not enhanced by the addition of lysozyme. Addition of holo-lactoferrin or cytochrome c diminished the antibacterial effect of apo-lactoferrin, whereas addition of BSA had no effect. Resistance to inhibition by lactoferrin was not related to the production of bacterial siderophores. |
| Databáze: | OpenAIRE |
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