Urotensin II receptor (UTR) exists in hyaline chondrocytes: A study of peripheral distribution of UTR in the African clawed frog, Xenopus laevis
| Autor: | Norifumi Konno, Minoru Uchiyama, Takao Mukuda, Kouhei Matsuda, Hiroyuki Kaiya, Yuya Fujii, Haruka Imae, Mikiya Miyazato |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Untranslated region Hyalin African clawed frog medicine.medical_specialty Peptide Hormones Urotensins Xenopus CHO Cells Urotensin-II receptor Receptors G-Protein-Coupled Xenopus laevis chemistry.chemical_compound Chondrocytes Cricetulus Endocrinology Cricetinae Internal medicine medicine Animals Tissue Distribution Amino Acid Sequence RNA Messenger Receptor biology Chinese hamster ovary cell Apical membrane biology.organism_classification Peptide Fragments Rats Cell biology Cartilage chemistry Calcium Female Animal Science and Zoology Urotensin-II Sequence Alignment |
| Zdroj: | General and Comparative Endocrinology. 185:44-56 |
| ISSN: | 0016-6480 |
| DOI: | 10.1016/j.ygcen.2013.01.015 |
| Popis: | Urotensin II (UII) and UII-related peptide (URP) exhibit diverse physiological actions including vasoconstriction, locomotor activity, osmoregulation, and immune response through UII receptor (UTR), which is expressed in the central nervous system and peripheral tissues of fish and mammals. In amphibians, only UII has been identified. As the first step toward elucidating the actions of UII and URP in amphibians, we cloned and characterized URP and UTR from the African clawed frog Xenopus laevis. Functional analysis showed that treatment of UII or URP with Chinese hamster ovary cells transfected with the cloned receptor increased the intracellular calcium concentration in a concentration-dependent manner, whereas the administration of the UTR antagonist urantide inhibited UII- or URP-induced Ca(2+) mobilization. An immunohistochemical study showed that UTR was expressed in the splenocytes and leukocytes isolated from peripheral blood, suggesting that UII and URP are involved in the regulation of the immune system. UTR was also localized in the apical membrane of the distal tubule of the kidney and in the transitional epithelial cells of the urinary bladder. This result supports the view that the UII/URP-UTR system plays an important role in osmoregulation of amphibians. Interestingly, immunopositive labeling for UTR was first detected in the chondrocytes of various hyaline cartilages (the lung septa, interphalangeal joint and sternum). The expression of UTR was also observed in the costal cartilage, tracheal cartilages, and xiphoid process of the rat. These novel findings probably suggest that UII and URP mediate the formation of the cartilaginous matrix. |
| Databáze: | OpenAIRE |
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