A Comparison of the Capacity of Mesenchymal Stromal Cells for Cartilage Regeneration Depending on Collagen-Based Injectable Biomimetic Scaffold Type
| Autor: | Alexandra D. Kirillova, Yulia B. Basok, Evgeniy A. Nemets, Ludmila A. Kirsanova, Alexey M. Grigoriev, Victor I. Sevastianov, Sergey V. Gautier, Anastasia M. Subbot |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: |
collagen
Science Adipose tissue Stimulation 02 engineering and technology General Biochemistry Genetics and Molecular Biology Article Glycosaminoglycan 03 medical and health sciences medicine articular cartilage automotive_engineering Ecology Evolution Behavior and Systematics 030304 developmental biology 0303 health sciences Decellularization Chemistry Regeneration (biology) Cartilage Mesenchymal stem cell Paleontology osteoarthrosis 021001 nanoscience & nanotechnology In vitro Cell biology medicine.anatomical_structure Space and Planetary Science decellularization hydrogel 0210 nano-technology mesenchymal stromal cells |
| Zdroj: | Life Volume 11 Issue 8 Life, Vol 11, Iss 756, p 756 (2021) |
| ISSN: | 2075-1729 |
| Popis: | Mesenchymal stromal cells (MSCs) have shown a high potential for cartilage repair. Collagen-based scaffolds are used to deliver and retain cells at the site of cartilage damage. The aim of the work was a comparative analysis of the capacity of the MSCs from human adipose tissue to differentiate into chondrocytes in vitro and to stimulate the regeneration of articular cartilage in an experimental model of rabbit knee osteoarthrosis when cultured on microheterogenic collagen-based hydrogel (МCH) and the microparticles of decellularized porcine articular cartilage (DPC). The morphology of samples was evaluated using scanning electron microscopy and histological staining methods. On the surface of the DPC, the cells were distributed more uniformly than on the MCH surface. On day 28, the cells cultured on the DPC produced glycosaminoglycans more intensely compared to the MCH with the synthesis of collagen type II. However, in the experimental model of osteoarthrosis, the stimulation of the cartilage regeneration was more effective when the MSCs were administered to the MCH carrier. The present study demonstrates the way to regulate the action of the MSCs in the area of cartilage regeneration: the MCH is more conducive to stimulating cartilage repair by the MSCs, while the DPC is an inducer for a formation of a cartilage-like tissue by the MSCs in vitro. |
| Databáze: | OpenAIRE |
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