Microanalysis of glycosaminoglycans
| Autor: | H.G. Weinstein, Bebe M. Baltrus, Dianne G. Lewandowski, Lawrence J. Blacik, Moira Breen, Paul A. Knepper |
|---|---|
| Rok vydání: | 1981 |
| Předmět: |
Electrophoresis
viruses Biophysics Biochemistry Microanalysis Glycosaminoglycan chemistry.chemical_compound Hyaluronidase medicine Animals Molecular Biology Glycosaminoglycans Cerebral Cortex chemistry.chemical_classification Nitrous acid Nuclease Chromatography biology Microchemistry Cell Biology Rats Enzyme chemistry biology.protein Densitometry medicine.drug |
| Zdroj: | Analytical Biochemistry. 113:416-422 |
| ISSN: | 0003-2697 |
| DOI: | 10.1016/0003-2697(81)90096-8 |
| Popis: | A sensitive and versatile method for the qualitative and quantitative determination of glycosaminoglycans (GAGs) is described. An enriched GAG fraction was subjected to nuclease enzyme treatment and to an appropriate sequence of GAG degrading enzymes—Streptomyces hyaluronidase, chondroitinase AC and ABC, and endo-β- d -galactosidase—and nitrous acid treatment. To determine the result of each degradative procedure, the remaining GAG polymers were subjected to cellulose acetate electrophoresis. The combination of sequential degradation and the monitoring of each step by electrophoresis and densitometry permitted the identification and quantitation of all the GAGs on a microscale basis. |
| Databáze: | OpenAIRE |
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