Regulation of platelet-activating factor receptor expression in human B cells and B cell lines
| Autor: | Yolande Richard, Zohair Mishal, Pierce Galanaud, Marek Rola-Pleszczynski, Dominique Treton, Cheikh M. Nguer, Yolène Thomas |
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| Rok vydání: | 1996 |
| Předmět: |
Receptors
Cell Surface Platelet Membrane Glycoproteins Biochemistry Cell Line Receptors G-Protein-Coupled Downregulation and upregulation Transcriptional regulation medicine Humans RNA Messenger Receptor Interleukin 4 B cell B-Lymphocytes Messenger RNA Chemistry Cell Membrane Organic Chemistry Cell Biology Molecular biology Up-Regulation Cell biology medicine.anatomical_structure Cell culture Interleukin-4 Platelet-activating factor receptor |
| Zdroj: | Lipids. 31:1051-1058 |
| ISSN: | 1558-9307 0024-4201 |
| DOI: | 10.1007/bf02522462 |
| Popis: | We extended our previous data regarding the modulation of human platelet-activating factor receptor (hPAF-R) expression on human B cell lines as well as normal B cells. First, we showed that hPAF-R mRNA was present in B cell lines expressing membrane hPAF-R, but was absent from cell lines devoid of hPAF-R. Second, enhanced hPAF-R membrane expression induced in IM9 line by IL4 was preceeded by hPAF-R mRNA accumulation that was detectable by 8 h and which peaked at 24 h. Similar results were observed for 10 nM platelet-activating factor treatment, which increased hPAF-R mRNA content up to 120% at 48 h, whereas hPAF-R membrane expression was up-regulated by 130%. Third, our data indicate that functional hPAF-R are expressed on resting, as well as on activated, B cells and that B cell activation is required for maintaining hPAF-R membrane and mRNA expression. Thus, in normal B cells, as well as in B cell lines, transcriptional regulation and/or messenger stability control hPAF-R expression. |
| Databáze: | OpenAIRE |
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