Dual microfluidic perifusion networks for concurrent islet perifusion and optical imaging

Autor: Adeola F. Adewola, Jose Oberholzer, Meirigeng Qi, Diana Gutierrez, Katie Kinzer, Joshua E. Mendoza-Elias, Yong Wang, Tricia A. Harvat, David T. Eddington, Dongyoung Lee
Rok vydání: 2011
Předmět:
Zdroj: Biomedical Microdevices. 14:7-16
ISSN: 1572-8781
1387-2176
DOI: 10.1007/s10544-011-9580-0
Popis: In this study, a new class of duplex microfluidic device is described that utilizes a dual perifusion network to simultaneously perform live-cell optical imaging of physiological activities and study insulin release kinetics on two islet populations. In addition, this device incorporates on-chip staggered herringbone mixers (SHMs) to increase mixing efficiency and facilitate the generation of user-defined chemical gradients. Using mouse islets, we simultaneously measure dynamic insulin release, changes in mitochondrial potentials, and calcium influx in responses to insulin secretagogues (glucose and tolbutamide), showing a high signal-to-noise ratio and spatiotemporal resolution of all measured parameters for both perifusion chambers. This system has many potential applications for studying β-cell physiology and pathophysiology, as well as for therapeutic drug screening. This dual perifusion device is not limited to islet studies and could easily be applied to other tissues and cells without major modifications.
Databáze: OpenAIRE
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