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Additional file 1: Table S1. Phytochemical constituents of methanolic leaves extract of Carica papaya and Psidium guajava. Table S2. Identified Compound from Carica papaya extract using HR-ESI-MS.Carica papaya extract shows 25% of alkaloids (Peptide, Amino acids), 5% of Glycoside, 10% lipids, 20% of Phenolic compounds (Aromatic Phenol, Quinone, Flavonoids), and 20% of Terpenes, 15% of Aliphatic Compounds (Fatty acids, alcohol and saturated, Unsaturated Alkenes) as well as 5% of other. We observed that both extracts have a higher percentage of alkaloids, Terpenes. Table S3. Identified Compound from Psidium guajava extract using HR-ESI-MS. The HR-ESI-MS data of Psidium guajava extract shows 40% of alkaloids (Peptide, Amino acids), 10% of Glycoside, 10% lipids, 10% of Phenolic compound (Aromatic Phenol, Quinone), and 10% of Terpenes, 15% of aliphatic Compounds (Fatty acids, alcohol and saturated, Unsaturated Alkenes) as well as 5% of other compounds were observed. Figure S1. Anti-HIV-1 activity of C. papaya and P. guajava in cell associated study. Dose-dependent inhibition of (A) HIV-1VB028 and (B) HIV-1UG070 replication in presence of C. papaya extract (0.125-1.500mg/ml). The effect of different concentrations of P. guajava extract (0.03125-1.500mg/ml) on (C) HIV-1VB028 and (D) HIV-1UG070 replication. Figure S2. Effects of C. papaya and P. guajava on HIV-1 suppression through cell-free assays. Percentage of inhibition observed in dose dependent manner for (A) HIV-1VB028 and (B) HIV-1UG070 in presence of C. papaya extract (0.125-1.500mg/ml). The effect of different concentrations of P. guajava extract (0.03125-1.500mg/ml) on (C) HIV-1VB028 and (D) HIV-1UG070 isolates. Figure S3. (A) ROS generator 15μM H2O2 (H2O2 generator) for 6 h served as positive control. (B) 15μM H2O2 (H2O2 generator) and 250U Catalase served as scavenger/inhibitor of ROS generation in the experiment. |
