Real-time multi-modality imaging of glioblastoma tumor resection and recurrence
| Autor: | Jose-Luiz Figueiredo, Christian T. Farrar, Deepak Bhere, Matthias Duebgen, Jordi Martinez-Quintanilla, Shawn Hingtgen, Khalid Shah |
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| Rok vydání: | 2012 |
| Předmět: |
Cancer Research
medicine.medical_specialty Pathology Time Factors Green Fluorescent Proteins Tumor resection Mice Nude Transfection Article Multi modality Mice Text mining Cell Line Tumor Glioma medicine Animals Humans Analysis of Variance medicine.diagnostic_test Brain Neoplasms business.industry Magnetic resonance imaging medicine.disease Xenograft Model Antitumor Assays Tumor Burden Platelet Endothelial Cell Adhesion Molecule-1 Disease Models Animal Diffusion Magnetic Resonance Imaging Ki-67 Antigen Neurology Oncology Luminescent Measurements Immunohistochemistry Neurology (clinical) Radiology Neoplasm Recurrence Local Glioblastoma business Preclinical imaging |
| Zdroj: | Journal of Neuro-Oncology. 111:153-161 |
| ISSN: | 1573-7373 0167-594X |
| Popis: | The lack of relevant pre-clinical animal models incorporating the clinical scenario of GBM resection and recurrence has contributed significantly to the inability to successfully treat the devastating brain tumor Glioblastoma multiforme (GBM). A multi-modality imaging approach that allows real-time assessment of tumor resection during surgery and non-invasive detection of post-operative tumor volumes is urgently needed. In this study, we report the development and implementation of an optical imaging and magnetic resonance imaging (MRI) approach to guide GBM resection during surgery and track tumor recurrence at multiple resolutions in mice. Intra-operative fluorescence-guided surgery allowed real-time monitoring of intracranial tumor removal and led to greater than 90% removal of established intracranial human GBM. The fluorescent signal clearly delineated tumor margins, residual tumor, and correlated closely with the clinically utilized fluorescence surgical marker 5-aminolevulinic acid/porphyrin. Post-operative non-invasive optical imaging and MRI confirmed near-complete tumor removal, which was further validated by correlated immunohistochemistry (IHC). Longitudinal non-invasive imaging and IHC showed rapid recurrence of multi-focal tumors that exhibited a faster growth rate and altered blood-vessel density compared to non-resected tumors. Surgical tumor resection significantly extended long-term survival, however mice ultimately succumbed to the recurrent GBM. This multi-modality imaging approach to GBM resection and recurrence in mice should provide an important platform for investigating multiple aspects of this deadly cancer and ultimately evaluating novel therapeutics. |
| Databáze: | OpenAIRE |
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