Cold-inducible RNA binding protein (CIRP), a novel XTcf-3 specific target gene regulates neural development in Xenopus
| Autor: | Martin Kunz, Doris Wedlich, Dagmar Fichtner, Dietmar Gradl, Stephanie van Venrooy |
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| Jazyk: | angličtina |
| Předmět: |
Embryo
Nonmammalian animal structures Lymphoid Enhancer-Binding Factor 1 RNA Stability Xenopus Transcription Factor 7-Like 1 Protein Nerve Tissue Proteins Xenopus Proteins TCF/LEF family Transcription Factor 3 Animals RNA Messenger lcsh:QH301-705.5 Gene Neural Plate biology Reverse Transcriptase Polymerase Chain Reaction Gene Expression Profiling Wnt signaling pathway Gene Expression Regulation Developmental RNA-Binding Proteins biology.organism_classification Molecular biology Antisense Elements (Genetics) Phenotype lcsh:Biology (General) embryonic structures TCF Transcription Factors Transcription Factor 7-Like 2 Protein Developmental biology Neural plate Neural development Function (biology) Research Article Developmental Biology |
| Zdroj: | BMC Developmental Biology, Vol 8, Iss 1, p 77 (2008) BMC Developmental Biology |
| ISSN: | 1471-213X |
| DOI: | 10.1186/1471-213x-8-77 |
| Popis: | Background As nuclear mediators of wnt/β-catenin signaling, Lef/Tcf transcription factors play important roles in development and disease. Although it is well established, that the four vertebrate Lef/Tcfs have unique functional properties, most studies unite Lef-1, Tcf-1, Tcf-3 and Tcf-4 and reduce their function to uniformly transduce wnt/β-catenin signaling for activating wnt target genes. In order to discriminate target genes regulated by XTcf-3 from those regulated by XTcf-4 or Lef/Tcfs in general, we performed a subtractive screen, using neuralized Xenopus animal cap explants. Results We identified cold-inducible RNA binding protein (CIRP) as novel XTcf-3 specific target gene. Furthermore, we show that knockdown of XTcf-3 by injection of an antisense morpholino oligonucleotide results in a general broadening of the anterior neural tissue. Depletion of XCIRP by antisense morpholino oligonucleotide injection leads to a reduced stability of mRNA and an enlargement of the anterior neural plate similar to the depletion of XTcf-3. Conclusion Distinct steps in neural development are differentially regulated by individual Lef/Tcfs. For proper development of the anterior brain XTcf-3 and the Tcf-subtype specific target XCIRP appear indispensable. Thus, regulation of anterior neural development, at least in part, depends on mRNA stabilization by the novel XTcf-3 target gene XCIRP. |
| Databáze: | OpenAIRE |
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