Channel Surface Patterning of Alternating Biomimetic Protein Combinations for Enhanced Microfluidic Tumor Cell Isolation
| Autor: | Marissa Gaskill, Ja Hye Myung, David T. Eddington, Cari Launiere, Gregory Czaplewski, Seungpyo Hong |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Surface Properties
Microfluidics chemistry.chemical_element Plasma protein binding Cell Separation Calcium Buffer (optical fiber) Article Analytical Chemistry Cell Line Tumor E-selectin Leukocytes Humans Chromatography Microchannel biology Cell adhesion molecule Elution Microfluidic Analytical Techniques Neoplastic Cells Circulating Immobilized Proteins chemistry biology.protein Biophysics E-Selectin Cell Adhesion Molecules Protein Binding |
| Popis: | Here, we report a new method for multicomponent protein patterning in a microchannel and also a technique for improving immunoaffinity-based circulating tumor cell (CTC) capture by patterning regions of alternating adhesive proteins using the new method. The first of two proteins, antiepithelial cell adhesion molecule (anti-EpCAM), provides the specificity for CTC capture. The second, E-selectin, increases CTC capture under shear. Patterning regions with and without E-selectin allows captured leukocytes, which also bind E-selectin and are unwanted impurities in CTC isolation, to roll a short distance and detach from the capture surface. This reduces leukocyte capture by up to 82%. The patterning is combined with a leukocyte elution step in which a calcium chelating buffer effectively deactivates E-selectin so that leukocytes may be rinsed away 60% more efficiently than with a buffer containing calcium. The alternating patterning of this biomimetic protein combination, used in conjunction with the elution step, reduces capture of leukocytes while maintaining a high tumor cell capture efficiency that is up to 1.9 times higher than the tumor cell capture efficiency of a surface with only anti-EpCAM. The new patterning technique described here does not require mask alignment and can be used to spatially control the immobilization of any two proteins or protein mixtures inside a sealed microfluidic channel. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|