In vitro systems toxicology-based assessment of the potential modified risk tobacco product CHTP 1.2 for vascular inflammation- and cytotoxicity-associated mechanisms promoting adhesion of monocytic cells to human coronary arterial endothelial cells
| Autor: | Dariusz Peric, Claudius Pak, Stefano Acali, David Bornand, Julia Hoeng, Emmanuel Guedj, Athanasios Kondylis, Nikolai V. Ivanov, Elodie Maluenda, Didier Goedertier, Alexandra Laurent, Ignacio Gonzalez Suarez, Karine Baumer, Remi Dulize, Diego Marescotti, Marco van der Toorn, Manuel C. Peitsch, Carine Poussin, Walter K. Schlage |
|---|---|
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Vasculitis Inflammation Pharmacology Toxicology medicine.disease_cause Monocytes Transcriptome 03 medical and health sciences 0302 clinical medicine Smoke Tobacco Toxicity Tests medicine Cell Adhesion Humans Cell adhesion Cytotoxicity Cells Cultured Chemistry Plant Extracts Monocyte General Medicine Coronary Vessels In vitro Endothelial stem cell 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis Endothelium Vascular medicine.symptom Oxidative stress Food Science |
| Zdroj: | Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association. 120 |
| ISSN: | 1873-6351 |
| Popis: | Cigarette smoking causes cardiovascular diseases. Heating tobacco instead of burning it reduces the amount of toxic compounds in the aerosol and may exert a reduced impact on health compared with cigarette smoke. Aqueous extract from the aerosol of a potential modified risk tobacco product, the Carbon Heated Tobacco Product (CHTP) 1.2, was compared in vitro with aqueous extract from the smoke of a 3R4F reference cigarette for its impact on the adhesion of monocytic cells to artery endothelial cells. Human coronary artery endothelial cells (HCAEC) were treated for 4 h with conditioned media from human monocytic Mono Mac 6 (MM6) cells exposed to CHTP1.2 or 3R4F extracts for 2 h or directly with those extracts freshly generated. In vitro monocyte-endothelial cell adhesion was measured concomitantly with inflammatory, oxidative stress, cytotoxicity, and death markers. Furthermore, transcriptomics analyses enabled to quantify the level of perturbation in HCAECs, and provide biological interpretation for the underlying molecular changes following exposure to 3R4F or CHTP1.2 extract. Our systems toxicology study demonstrated that approximately 10–15-fold higher concentrations of the CHTP 1.2 aerosol extract were needed to elicit similar effects as the 3R4F smoke extract on cardiovascular disease-relevant inflammation and cytotoxicity-related mechanisms and markers investigated in vitro . |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect