| Autor: |
Christian Schöne, Anja Poehlein, Nico Jehmlich, Norman Adlung, Rolf Daniel, Martin von Bergen, Silvan Scheller, Michael Rother |
| Přispěvatelé: |
Technische Universität Dresden, University of Göttingen, Helmholtz Centre for Environmental Research, Department of Bioproducts and Biosystems, Aalto-yliopisto, Aalto University |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Schone, C, Poehlein, A, Jehmlich, N, Adlung, N, Daniel, R, von Bergen, M, Scheller, S & Rother, M 2022, ' Deconstructing Methanosarcina acetivorans into an acetogenic archaeon ', Proceedings of the National Academy of Sciences of the United States of America, vol. 119, no. 2, e2113853119 . https://doi.org/10.1073/pnas.2113853119 |
| Popis: |
Funding Information: ACKNOWLEDGMENTS. We thank Seigo Shima (Marburg) and Uwe Deppen-meier (Bonn) for generously providing CoM-S-S-CoB, Johannes Finke (Frankfurt) for help during cloning procedures, and Ivan Berg (Mu€nster) for critical reading of the manuscript. M.V. and N.J. acknowledge support from the Helmholtz Centre for Environmental Research for the ProMetheus platform for proteomics and metabolomics. This work was supported by grants from the Graduate Academy of the Technische Universita€t Dresden to C.S., Novo Nordisk Foundation Grant NNF19OC0055464 to N.A., and Academy of Finland Grant 326020 to S.S. Funding Information: ACKNOWLEDGMENTS. We thank Seigo Shima (Marburg) and Uwe Deppenmeier (Bonn) for generously providing CoM-S-S-CoB, Johannes Finke (Frankfurt) for help during cloning procedures, and Ivan Berg (Munster) ? for critical reading of the manuscript. M.V. and N.J. acknowledge support from the Helmholtz Centre for Environmental Research for the ProMetheus platform for proteomics and metabolomics. This work was supported by grants from the Graduate Academy of the Technische Universitat ? Dresden to C.S., Novo Nordisk Foundation Grant NNF19OC0055464 to N.A., and Academy of Finland Grant 326020 to S.S. Publisher Copyright: © 2022 National Academy of Sciences. All rights reserved. The reductive acetyl-coenzyme A (acetyl-CoA) pathway, whereby carbon dioxide is sequentially reduced to acetyl-CoA via coenzyme-bound C1 intermediates, is the only autotrophic pathway that can at the same time be the means for energy conservation. A conceptually similar metabolism and a key process in the global carbon cycle is methanogenesis, the biogenic formation of methane. All known methanogenic archaea depend on methanogenesis to sustain growth and use the reductive acetyl-CoA pathway for autotrophic carbon fixation. Here, we converted a methanogen into an acetogen and show that Methanosarcina acetivorans can dispense with methanogenesis for energy conservation completely. By targeted disruption of the methanogenic pathway, followed by adaptive evolution, a strain was created that sustained growth via carbon monoxide–dependent acetogenesis. A minute flux (less than 0.2% of the carbon monoxide consumed) through the methane-liberating reaction remained essential, indicating that currently living methanogens utilize metabolites of this reaction also for anabolic purposes. These results suggest that the metabolic flexibility of methanogenic archaea might be much greater than currently known. Also, our ability to deconstruct a methanogen into an acetogen by merely removing cellular functions provides experimental support for the notion that methanogenesis could have evolved from the reductive acetyl-coenzyme A pathway. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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