cAMP measurements with FRET-based sensors in excitable cells
| Autor: | Katy L. Everett, Dermot M.F. Cooper |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
Chemistry
Green Fluorescent Proteins Hydrogen-Ion Concentration Second Messenger Systems Biochemistry Fluorescence Recombinant Proteins Cell biology Förster resonance energy transfer Second messenger system Cyclic AMP Fluorescence Resonance Energy Transfer Animals Humans Single-Cell Analysis Cyclic AMP metabolism |
| Zdroj: | Biochemical Society Transactions. 40:179-183 |
| ISSN: | 1470-8752 0300-5127 |
| DOI: | 10.1042/bst20110616 |
| Popis: | The development of FRET (fluorescence resonance energy transfer)-based sensors for measuring cAMP has opened the door to sophisticated insights into single-cell cAMP dynamics. cAMP can be measured in distinct cell populations and even in distinct microdomains within cells. However, there is still only limited information on cAMP dynamics in excitable cells, particularly as a function of the activity of voltage-gated Ca2+ channels. A major reason for this is the pH shifts that can occur in excitable cells and their effects on fluorescent proteins. |
| Databáze: | OpenAIRE |
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