circCCT3 Modulates Vascular Endothelial Growth Factor A and Wnt Signaling to Enhance Colorectal Cancer Metastasis Through Sponging miR-613
| Autor: | Mengjia Dong, Qianyi Tao, Weiliang Li, Youqi Xu, Wenjing Bu, Guang Cao, Yao Xu, Xia Wang, Zhen Fang, Xiaodong Wang |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Vascular Endothelial Growth Factor A
0301 basic medicine Colorectal cancer Kaplan-Meier Estimate Biology Metastasis Wnt3 Protein 03 medical and health sciences 0302 clinical medicine Western blot Annexin Cell Line Tumor Sequence Homology Nucleic Acid Genetics medicine Humans Neoplasm Metastasis 3' Untranslated Regions Wnt Signaling Pathway neoplasms Molecular Biology Base Sequence medicine.diagnostic_test Wnt signaling pathway Cancer RNA Circular Cell Biology General Medicine HCT116 Cells medicine.disease digestive system diseases Gene Expression Regulation Neoplastic MicroRNAs Vascular endothelial growth factor A 030104 developmental biology Apoptosis 030220 oncology & carcinogenesis Cancer research Colorectal Neoplasms Chaperonin Containing TCP-1 |
| Zdroj: | DNA and Cell Biology. 39:118-125 |
| ISSN: | 1557-7430 1044-5498 |
| DOI: | 10.1089/dna.2019.5139 |
| Popis: | Colorectal cancer (CRC) has been suggested to be one of the leading cancer types all over the world. Till now, the molecular mechanism by which circCCT3 regulates CRC remains to be clarified. To detect mRNA and protein levels of various genes, Reverse Transcription-quantitative PCR and western blot were used in our study. Luciferase reporter assay was utilized to probe direct interaction between genes. We used transwell assay to assess the invasion ability of CRC cells. For apoptosis detection, immunofluorescence of CRC cells by Annexin V staining was performed. We carried out bioinformatic analysis to show higher expression of circCCT3 in human clinical CRC tumors. Low level of circCCT3 was closely associated with higher disease-free survival of CRC patients. Moreover, we found that circCCT3 was linked to advanced stage of CRC. miR-613 is the target of circCCT3 and responsible for circCCT3-modulated invasion and apoptosis of CRC cells. In addition, we identified WNT3 and vascular endothelial growth factor A (VEGFA) as downstream effectors of miR-613 in CRC cells. WNT3 and VEGFA overexpression resulted in partial rescue of miR-613-mediated phenotypes of CRC cells. In conclusion, we propose that circCCT3 contributes to CRC metastasis via miR-613/WNT3 or miR-613/VEGFA, promoting the development of therapeutical approaches for treating CRC. |
| Databáze: | OpenAIRE |
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