Functional similarity of modified cascade impactor to deposit drug particles on cells
| Autor: | Jonathan Cheong, Ajit S. Narang, Virender Kumar, Jerry Tso, Ram I. Mahato, Jitender Bariwal |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Drug
Drug Compounding media_common.quotation_subject Pharmaceutical Science Bronchi 02 engineering and technology 030226 pharmacology & pharmacy Permeability Cell Line 03 medical and health sciences 0302 clinical medicine In vivo Administration Inhalation Monolayer Humans Technology Pharmaceutical Particle Size Aerosolization media_common Aerosols Chemistry Biological Transport Epithelial Cells Equipment Design 021001 nanoscience & nanotechnology Aerosol Deposition (aerosol physics) Drug delivery Biophysics Fluticasone 0210 nano-technology Particle deposition |
| Zdroj: | International Journal of Pharmaceutics. 583:119404 |
| ISSN: | 0378-5173 |
| DOI: | 10.1016/j.ijpharm.2020.119404 |
| Popis: | Pulmonary drug delivery is a non-invasive and effective route for local or systemic drug administration. Despite several products in the market, the mechanism of drug absorption from the lungs is not well understood. An in vitro model for aerosol deposition and transport across epithelia that uses particle deposition may be a good predictor of and help understand in vivo drug disposition. The objective of this study was to examine the uptake of HFA fluticasone (Flovent HFA) particles at various stages of the Next Generation Impactor (NGI) by human Calu-3 cell line derived from human bronchial respiratory epithelial cell monolayer. Particles were directly deposited on Calu-3 cells incorporated onto stages 3, 5, and 7 of the NGI at the air-liquid interface (ALI). We modified the NGI apparatus to allow particle deposition directly on cells and determined the in vitro deposition characteristics using modified NGI. Particles of different size ranges showed different in vitro epithelial transport rates. This study highlights the need to develop in vitro test systems to determine the deposition of aerosol particles on cell monolayers by simultaneously considering aerodynamic properties. |
| Databáze: | OpenAIRE |
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