Stoichiometric Relationship between Na+ Ions Transported and Glucose Consumed in Human Erythrocytes: Bayesian Analysis of 23Na and 13C NMR Time Course Data
| Autor: | Philip W. Kuchel, Gemma A. Figtree, Bogdan E. Chapman, Max Puckeridge, Stuart M. Grieve, Arthur D. Conigrave |
|---|---|
| Rok vydání: | 2013 |
| Předmět: |
Erythrocytes
Magnetic Resonance Spectroscopy Sodium Sodium-Potassium-Exchanging ATPase Inorganic chemistry Ionophore Sodium Ionophores Biophysics chemistry.chemical_element Models Biological Ouabain chemistry.chemical_compound medicine Humans Monensin Ion transporter Carbon Isotopes Chromatography Ion Transport Bayes Theorem Nuclear magnetic resonance spectroscopy respiratory system Red blood cell Kinetics medicine.anatomical_structure Glucose chemistry Cell Biophysics Sodium Isotopes medicine.drug |
| Zdroj: | Biophysical Journal. 104(8):1676-1684 |
| ISSN: | 0006-3495 |
| DOI: | 10.1016/j.bpj.2013.03.019 |
| Popis: | We examined the response of Na(+),K(+)-ATPase (NKA) to monensin, a Na(+) ionophore, with and without ouabain, an NKA inhibitor, in suspensions of human erythrocytes (red blood cells). A combination of (13)C and (23)Na NMR methods allowed the recording of intra- and extracellular Na(+), and (13)C-labeled glucose time courses. The net influx of Na(+) and the consumption of glucose were measured with and without NKA inhibited by ouabain. A Bayesian analysis was used to determine probability distributions of the parameter values of a minimalist mathematical model of the kinetics involved, and then used to infer the rates of Na(+) transported and glucose consumed. It was estimated that the numerical relationship between the number of Na(+) ions transported by NKA per molecule of glucose consumed by a red blood cell was close to the ratio 6.0:1.0, agreeing with theoretical prediction. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|