Selective Expansion of Skeletal Muscle Stem Cells from Bulk Muscle Cells in Soft Three-Dimensional Fibrin Gel
| Autor: | Xin Wang, Wen Shu Wu, Yalu Zhou, Yuanfan Hong, Jeffrey Mosenson, Furen Wu, Pei Zhu, Gajendra S. Shekhawat |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Satellite Cells Skeletal Muscle Muscle Fibers Skeletal Muscle disorder Fibrin Mice 03 medical and health sciences Translational Research Articles and Reviews In vivo Satellite cells Muscle stem cells medicine Animals Myocyte Ex vivo expansion biology Stem Cells Skeletal muscle Cell Differentiation Cell Biology General Medicine Cell sorting Cell biology 030104 developmental biology medicine.anatomical_structure Immunology biology.protein Three‐dimensional fibrin gel Stem cell Gels Myogenic progenitor cells Tissue‐Specific Progenitor and Stem Cells Developmental Biology |
| Zdroj: | Stem Cells Translational Medicine |
| ISSN: | 2157-6580 2157-6564 |
| DOI: | 10.1002/sctm.16-0427 |
| Popis: | Muscle stem cells (MuSCs) exhibit robust myogenic potential in vivo, thus providing a promising curative treatment for muscle disorders. Ex vivo expansion of adult MuSCs is highly desired to achieve a therapeutic cell dose because of their scarcity in limited muscle biopsies. Sorting of pure MuSCs is generally required for all the current culture systems. Here we developed a soft three-dimensional (3D) salmon fibrin gel culture system that can selectively expand mouse MuSCs from bulk skeletal muscle preparations without cell sorting and faithfully maintain their regenerative capacity in culture. Our study established a novel platform for convenient ex vivo expansion of MuSCs, thus greatly advancing stem cell-based therapies for various muscle disorders. |
| Databáze: | OpenAIRE |
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