Intrahepatic hepatitis C virus RNA quantification in microdissected hepatocytes
| Autor: | Valérie Thiers, Rosella Tuveri, Jean Baptiste Trabut, Stanislas Pol, Anais Vallet, Brigitte Le Bail, Bertrand Nalpas, Christian Bréchot, Françoise Carnot, Oona Delpuech, Giorgio Ballardini, Giovanna Vona, Danielle Canioni |
|---|---|
| Přispěvatelé: | Carcinogenèse Hépatique et Virologie Moléculaire, Institut National de la Santé et de la Recherche Médicale (INSERM), Centre d'infectiologie Necker-Pasteur [CHU Necker], Institut Pasteur [Paris] (IP)-CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), CHU Necker - Enfants Malades [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Service d'anatomie pathologique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Alma Mater Studiorum Università di Bologna [Bologna] (UNIBO), Hôpital Pellegrin, CHU Bordeaux [Bordeaux]-Groupe hospitalier Pellegrin, The study was supported by grants from Institut National de la Santé et de la Recherche Médicale (INSERM), from the Agence Nationale de la Recherche sur le Sida (ANRS, grant CNR-2001-14), from the Association pour la Recherche contre le Cancer (ARC) and from the European Community. Giovanna Vona and Oona Delpuech were supported by ANRS. |
| Rok vydání: | 2003 |
| Předmět: |
Male
Pathology Cirrhosis [SDV]Life Sciences [q-bio] Hepacivirus MESH: Base Sequence medicine.disease_cause Virus Replication MESH: Hepatocytes Liver disease MESH: Hepacivirus MESH: Aged MESH: Middle Aged biology Hepatitis C virus Liver cell Dissection virus diseases Middle Aged MESH: Hepatitis C Chronic MESH: RNA Viral RNA Viral Female Viral disease Viral load Adult medicine.medical_specialty Intrahepatic HCV MESH: Dissection medicine Humans Viremia MESH: Viremia Aged Hepatitis MESH: Humans Hepatology Base Sequence MESH: Virus Replication MESH: Adult Hepatitis C Antibodies Hepatitis C Chronic medicine.disease biology.organism_classification Virology MESH: Male digestive system diseases MESH: DNA Viral MESH: Hepatitis C Antibodies DNA Viral Hepatocytes Laser microdissection MESH: Female |
| Zdroj: | Journal of Hepatology Journal of Hepatology, 2004, 40 (4), pp.682-688. ⟨10.1016/j.jhep.2003.12.012⟩ |
| ISSN: | 0168-8278 1600-0641 |
| DOI: | 10.1016/j.jhep.2003.12.012⟩ |
| Popis: | Background/Aims: Debate continues on whether serum and intrahepatic HCV viral loads are correlated and if HCV viral load correlates with the severity of liver disease. These difficulties may at least in part be linked to liver cell heterogeneity, when total liver extracts from HCV-infected individuals are tested for HCV RNA quantification. We have therefore investigated the feasibility of quantifying HCV replication using a laser-based microdissection technique. Methods: We compared the results with those obtained for serum HCV RNA quantification and immunochemistry in the case of HCV antigen detection in the liver. Twenty-one HCV-positive patients with chronic active hepatitis ( n =10) or cirrhosis ( n =11) were analyzed. Results: A positive correlation ( P =0.0019) was observed between HCV RNA quantifications in sera and microdissected cells. Immunohistochemistry demonstrated that HCV antigen hepatocytes were randomly distributed within liver lobules. Their percentage varied in different patients (0–40%), but did not correlate with the HCV viral load. Conclusions: We have designed a sensitive methodology to evaluate the intrahepatic HCV viral load by combining a standardized RNA quantification method with microdissected hepatocytes from frozen liver needle biopsies. Our results directly demonstrate a positive correlation between serum and intrahepatic viral loads, which therefore provides a reliable reflection of intrahepatic HCV replication. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect