| Autor: |
Gabriela Figueroa-Miranda, Yuanying Liang, Mohit Suranglikar, Matthias Stadler, Nagesh Samane, Marcel Tintelott, Young Lo, Julian A. Tanner, Xuan T. Vu, Joachim Knoch, Sven Ingebrandt, Andreas Offenhäusser, Vivek Pachauri, Dirk Mayer |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Biosensors and bioelectronics 208, 114219 (2022). doi:10.1016/j.bios.2022.114219 |
| Popis: |
Despite significant eradication efforts, malaria remains a persistent infectious disease with high mortality due to the lack of efficient point-of-care (PoC) screening solutions required to manage low-density asymptomatic parasitemia. In response, we demonstrate a quantitative electrical biosensor based on system-integrated two-dimensional field-effect transistors (2DBioFETs) of reduced graphene oxide (rGO) as transducer for high sensitivity screening of the main malaria biomarker, Plasmodium falciparum lactate dehydrogenase (PfLDH). The 2DBioFETs were biofunctionalized with pyrene-modified 2008s aptamers as specific PfLDH receptors. While we systematically optimize biosensor interface for optimal performance, aptamer-protein transduction at 2DBioFETs is elucidated based on delineation of charge and capacitance in an updated analytical model for two-dimensional rGO/biofunctional layer/electrolyte (2DiBLE) interfaces. Our 2DBioFET-aptasensors display a limit-of-detection down to 0.78 fM (0.11 pg/mL), dynamic ranges over 9 orders of magnitude (subfemto to submicromolar), high sensitivity, and selectivity in human serum validating their diagnostic potential as rapid PoC tests for malarial management. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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