Hematological Changes in Dogs with Visceral Leishmaniasis Are Associated with Increased IFN-γ and TNF Gene Expression Levels in the Bone Marrow
Autor: | Deborah Bittencourt Mothé Fraga, Eugenia Carrillo, Washington Luis Conrado dosSantos, Valter dos Anjos Almeida, Isadora dos Santos Lima, Javier Moreno |
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Přispěvatelé: | Instituto de Salud Carlos III, National Council for Scientific and Technological Development (Brasil), Oswaldo Cruz Foundation |
Rok vydání: | 2021 |
Předmět: |
0301 basic medicine
Microbiology (medical) medicine.medical_specialty bone marrow QH301-705.5 030231 tropical medicine Microbiology Article Leishmania infantum 03 medical and health sciences 0302 clinical medicine Virology Internal medicine parasitic diseases medicine semidomiciled dogs Bone marrow Leukocytosis Biology (General) Hematology biology business.industry hematology medicine.disease biology.organism_classification cytokines Histiocytosis Red blood cell 030104 developmental biology medicine.anatomical_structure Visceral leishmaniasis Immunology Cytokines Tumor necrosis factor alpha medicine.symptom business Semidomiciled dogs |
Zdroj: | Microorganisms Microorganisms, Vol 9, Iss 1618, p 1618 (2021) Volume 9 Issue 8 Repisalud Instituto de Salud Carlos III (ISCIII) |
ISSN: | 2076-2607 |
Popis: | Visceral leishmaniasis is associated with a variety of hematological abnormalities. In this study, we correlated the hematological changes in the peripheral blood of dogs naturally infected with Leishmania infantum (L. infantum) with the distribution of cell lineages and cytokine gene expression patterns in the bone marrow. Samples from 63 naturally semidomiciled dogs living in an endemic area of visceral leishmaniasis were analyzed. L. infantum infection was detected in 50 dogs (79.3%). Among those, 18 (32%) had positive splenic cultures and showed more clinical signs. They also had lower red blood cell counts and leukocytosis with an increased number of neutrophils and monocytes in peripheral blood compared to dogs negative to this test. L. infantum DNA was detected in the bone marrow of 8/14 dogs with positive splenic culture. Dogs with L. infantum infection in the bone marrow presented with histiocytosis (p = 0.0046), fewer erythroid cell clusters (p = 0.0127) and increased gene expression levels of IFN-γ (p = 0.0015) and TNF (p = 0.0091). The data shown herein suggest that inflammatory and cytokine gene expression changes in bone marrow may contribute to the peripheral blood hematological changes observed in visceral leishmaniasis. V.A. was supported by a scholarship from CNPq (Conselho Nacional de Desenvolvimento Científico e Tecnológico), FIOCRUZ and Instituto de Salud Carlos III (ISC-III). Sí |
Databáze: | OpenAIRE |
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