Adenoviral-expressed recombinant granulocyte monocyte colony-stimulating factor (GM-CSF) enhances protective immunity induced by inactivated Newcastle Disease Virus (NDV) vaccine
| Autor: | Xinglong Wang, Xiangwei Wang, Zen H. Lu, Yanqing Jia, Zengqi Yang, Qinqsong Han, Chongyang Wang |
|---|---|
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
animal structures Newcastle Disease viruses medicine.medical_treatment Newcastle disease virus Immunopotentiator Biology Antibodies Viral Newcastle disease Virus Adenoviridae Microbiology 03 medical and health sciences 0302 clinical medicine Immune system Adjuvants Immunologic Virology medicine Animals 030212 general & internal medicine Viral shedding Pharmacology Drug Carriers Histocompatibility Antigens Class II Granulocyte-Macrophage Colony-Stimulating Factor Viral Vaccines Hemagglutination Inhibition Tests biology.organism_classification Survival Analysis Recombinant Proteins Vaccination 030104 developmental biology Vaccines Inactivated Leukocytes Mononuclear Interferons Interleukin-4 Chickens Adjuvant Viral load Spleen |
| Zdroj: | Antiviral Research. 144:322-329 |
| ISSN: | 0166-3542 |
| DOI: | 10.1016/j.antiviral.2017.07.004 |
| Popis: | Although vaccination has been hugely successful in protecting birds against infection by the New castle disease virus (NDV), newly-emerged highly virulent strains have been found to overcome established immune protection and threaten the poultry industry. The need to improve the immunization efficacy is, therefore, urgent. Here, we tested the potential immunostimulatory adjuvant activity of the adenoviral-expressed recombinant chicken granulocyte monocyte colony stimulating factor (rchGM-CSF) in an inactivated Newcastle Disease Virus (NDV) vaccine. 126 commercial layer chicks, divided into six groups, were first vaccinated at day 7, followed by a subsequent boost and later an intramuscular challenge at day 21 and 35 respectively. rchGM-CSF expressed by adenovirus raised NDV-specific hemagglutinin-inhibition (HI) titers from 10 to 12 (log2) and significantly upregulated the production of interferon α/β/γ (IFN-α/β/γ), interleukin-4 (IL-4) and major histocompatibility complex II (MHC-II) in spleens. Crucially, chicks inoculated with the inactivated NDV vaccine plus the rchGM-CSF adjuvant displayed only mild clinical signs, lower tissue viral loads, fewer tissue lesions, and decreased mortality and viral shedding than those in the group immunized with the vaccine alone. Our present work has demonstrated that chicken GM-CSF may act as an enhancer in the orchestration of host immune responses induced by the inactivated NDV vaccine. The molecule, expressed by an adenovirus, has the potential to be used as an immune adjuvant to improve protection by NDV vaccination. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect