Icariin-Functionalized Nanodiamonds to Enhance Osteogenic Capacity In Vitro
| Autor: | Kyeongsoon Park, Hak Jun Kim, Sung Hyun Noh, Ji Seon Min, Somang Choi, Han-Saem Jo, Chae Ouk Lim, Sungeun Kim |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Biocompatibility
General Chemical Engineering icariin 02 engineering and technology Article lcsh:Chemistry 03 medical and health sciences chemistry.chemical_compound stomatognathic system General Materials Science Osteopontin MC3T3-E1 cells 030304 developmental biology 0303 health sciences biology 021001 nanoscience & nanotechnology Molecular biology In vitro RUNX2 Collagen type I alpha 1 chemistry lcsh:QD1-999 nanodiamonds Drug delivery biology.protein cardiovascular system Alkaline phosphatase 0210 nano-technology Icariin osteogenesis promotion |
| Zdroj: | Nanomaterials Volume 10 Issue 10 Nanomaterials, Vol 10, Iss 2071, p 2071 (2020) |
| ISSN: | 2079-4991 |
| Popis: | Nanodiamonds (NDs) have been used as drug delivery vehicles due to their low toxicity and biocompatibility. Recently, it has been reported that NDs have also osteogenic differentiation capacity. However, their capacity using NDs alone is not enough. To significantly improve their osteogenic activity, we developed icariin (ICA)-functionalized NDs (ICA-NDs) and evaluated whether ICA-NDs enhance their in vitro osteogenic capacity. Unmodified NDs and ICA-NDs showed nanosized particles that were spherical in shape. The ICA-NDs achieved a prolonged ICA release for up to 4 weeks. The osteogenic capacities of NDs, ICA (10 &mu g)-NDs, and ICA (50 &mu g)-NDs were demonstrated by alkaline phosphatase (ALP) activity calcium content and mRNA gene levels of osteogenic-related markers, including ALP, runt-related transcript factor 2 (RUNX2), collagen type I alpha 1 (COL1A1), and osteopontin (OPN). In vitro cell studies revealed that ICA (50 &mu g)-ND-treated MC3T3-E1 cells greatly increased osteogenic markers, including ALP, calcium content, and mRNA gene levels of osteogenic-related markers, including ALP, RUNX2, COL1A1, and OPN compared to ICA (10 &mu g)-NDs or ND-treated cells. These our data suggest that ICA-NDs can promote osteogenic capacity. |
| Databáze: | OpenAIRE |
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