An efficient quantitation method of next-generation sequencing libraries by using MiSeq sequencer

Autor:	Masao Nagasaki, Xiaoqing Pan, Jun Yasuda, Shin Ito, Kaoru Tsuda, Masayuki Yamamoto, Junji Yokozawa, Fumiki Katsuoka
Rok vydání:	2014
Předmět:	Genomic Library Computer science Biophysics Illumina miseq High-Throughput Nucleotide Sequencing Cell Biology Computational biology Sequence Analysis DNA Molecular Biology Biochemistry Molecular biology DNA sequencing
Zdroj:	Analytical biochemistry. 466
ISSN:	1096-0309
Popis:	Library quantitation is a critical step to obtain high data output in Illumina HiSeq sequencers. Here, we introduce a library quantitation method that uses the Illumina MiSeq sequencer designated as quantitative MiSeq (qMiSeq). In this procedure, 96 dual-index libraries, including control samples, are denatured, pooled in equal volume, and sequenced by MiSeq. We found that relative concentration of each library can be determined based on the observed index ratio and can be used to determine HiSeq run condition for each library. Thus, qMiSeq provides an efficient way to quantitate a large number of libraries at a time.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d4e8eeceb18b7e10bcc821f1b50da9d https://pubmed.ncbi.nlm.nih.gov/25173513 Zobrazit plný text záznamu Full Text from ScienceDirect