Flexivirga oryzae sp. nov., isolated from soil of a rice paddy, and emended description of the genus Flexivirga Anzai et al. 2012
| Autor: | Hyo Jung Lee, Jong Woo Hyeon, Che Ok Jeon, Sang-Ho Choi, Hye Rim Kim, Sang Eun Jeong |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
DNA Bacterial Sequence analysis 030106 microbiology Peptidoglycan Oryza Microbiology Actinobacteria 03 medical and health sciences chemistry.chemical_compound RNA Ribosomal 16S Botany Actinomycetales Republic of Korea Ecology Evolution Behavior and Systematics Phospholipids Phylogeny Soil Microbiology Base Composition biology Fatty Acids Vitamin K 2 General Medicine Sequence Analysis DNA Ribosomal RNA biology.organism_classification 16S ribosomal RNA Bacterial Typing Techniques chemistry Chemotaxonomy |
| Zdroj: | International journal of systematic and evolutionary microbiology. 67(2) |
| ISSN: | 1466-5034 |
| Popis: | A Gram-staining-positive, strictly aerobic bacterium, designated R1T, was isolated from a rice paddy in South Korea. Cells were non-motile cocci showing oxidase-negative and catalase-positive activities. Growth of strain R1T was observed at 10–37 °C (optimum, 30 °C) and pH 5.0–9.0 (optimum, pH 7.0). Strain R1T contained iso-C16 : 0, summed feature 9 (comprising iso-C17 : 1ω9c/10-methyl C16 : 0), anteiso-C17 : 0, iso-C17 : 0 and anteiso-C17 : 1ω9c as the major fatty acids and MK-8 (H4) and MK-8 (H6) as the isoprenoid quinones. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, three unidentified phospholipids, four unidentified aminolipids, one unidentified glycolipid and four unidentified lipids. The peptidoglycan type was A4α with an l-Lys−l-Ser2−d-Glu interpeptide bridge containing a Gly residue. The G+C content of the genomic DNA was 64.5 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain R1T was most closely related to the members of the genus Flexivirga , Flexivirga endophytica YIM 7505T (97.7 %), Flexivirga luteaTBS-100T (97.5 %) and Flexivirga alba ST13T (97.2 %). On the basis of phenotypic, chemotaxonomic and molecular properties, it is clear that strain R1T represents a novel species of the genus Flexivirga , for which the name Flexivirga oryzae sp. nov. is proposed. The type strain is R1T (=KACC 18597T=JCM 31060T). An emended description of the genus Flexivirga is also proposed. |
| Databáze: | OpenAIRE |
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