Dynamic Measurements of Bovine Serum Albumin Aggregation Using Small Angle Neutron Scattering

Autor:	Austerberry, James I, Belton, Daniel J
Rok vydání:	2018
Předmět:	education.field_of_study biology Scattering Population Burst phase Protein aggregation Neutron scattering Small-angle neutron scattering chemistry.chemical_compound Monomer chemistry biology.protein Biophysics Bovine serum albumin education
DOI:	10.1101/368092
Popis:	The rapid and complex nature of protein aggregation makes the identification of aggregation mechanisms and their precursors challenging. Here we demonstrate the novel use of small-angle neutron scattering to perform dynamic real-time measurement and analysis of protein aggregation. Changes in bovine serum albumin monomer population and aggregate size are identified at several isothermal temperatures. Kratky plots indicate that the aggregation of BSA occurs through the partial unfolding of the monomer. Dual population modelling of the scattering data indicates that the protein nucleates and grows through a two stage mechanism; a rapid burst phase and a slower growth phase. Both stages are observed to follow Arrhenius behaviour between 70-80 °C.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=doi_dedup___::1d1d346bd524fc9b85b857e349bd9afd https://doi.org/10.1101/368092 Zobrazit plný text záznamu