Novel mouse monoclonal antibodies specifically recognizing β-(1→3)-D-glucan antigen
| Autor: | Yury E. Tsvetkov, D. V. Yashunsky, Ivan K. Baykov, Yana A. Khlusevich, Alexander A. Karelin, Vadim B. Krylov, Sarah Sze Wah Wong, Vishukumar Aimanianda, Jean-Paul Latgé, Alevtina V. Bardashova, Nina V. Tikunova, Nikolay E. Nifantiev, Andrey L. Matveev, Ljudmila A. Emelyanova |
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| Přispěvatelé: | Institute of Chemical Biology and Fundamental Medicine [Novosibirsk, Russia] (ICBFM SB RAS), Siberian Branch of the Russian Academy of Sciences (SB RAS), ND Zelinsky Institute of Organic Chemistry [Moscow, Russia], Novosibirsk State University (NSU), Aspergillus, Institut Pasteur [Paris], Mycologie moléculaire - Molecular Mycology, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Franco-German ANR-DFG (ANR-16-CE92-0031-01 AFUINTERACT to V. Kumar, (http://www.agence-nationale-recherche.fr) and the Franco-Indian CEFIPRA (Project N° 5403-1 to V. Kumar) grants., Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], Institut Pasteur [Paris] (IP), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), ANR-16-CE92-0031,EpiFUS,Rôle de FUS dans la régulation des modifications épigénétiques : conséquences pour la sclérose latérale amyotrophique et la démence frontotemporale(2016) |
| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0301 basic medicine
Antifungal Agents beta-Glucans [SDV]Life Sciences [q-bio] MESH: Drug Synergism Aspergillus fumigatus MESH: Antibodies Monoclonal Mice Cell Wall Candida albicans MESH: Animals Bovine serum albumin Fluconazole MESH: Treatment Outcome chemistry.chemical_classification MESH: Microbial Sensitivity Tests Multidisciplinary biology MESH: beta-Glucans Candidiasis Antibodies Monoclonal Drug Synergism Oligosaccharide MESH: Candidiasis 3. Good health Treatment Outcome Biochemistry Biotinylation Medicine MESH: Fluconazole [SDV.IMM]Life Sciences [q-bio]/Immunology Drug Therapy Combination Female MESH: Aspergillus fumigatus Research Article Antigens Fungal medicine.drug_class Science 030106 microbiology Microbial Sensitivity Tests Monoclonal antibody 03 medical and health sciences MESH: Cell Wall medicine Animals Humans MESH: Mice MESH: Antigens Fungal MESH: Humans MESH: Candida albicans [SDV.IMM.IMM]Life Sciences [q-bio]/Immunology/Immunotherapy biology.organism_classification MESH: Antifungal Agents In vitro Disease Models Animal MESH: Drug Therapy Combination 030104 developmental biology chemistry biology.protein Hybridoma technology MESH: Disease Models Animal MESH: Female |
| Zdroj: | PLoS ONE PLoS ONE, Public Library of Science, 2019, 14 (4), pp.e0215535. ⟨10.1371/journal.pone.0215535⟩ PLoS ONE, 2019, 14 (4), pp.e0215535. ⟨10.1371/journal.pone.0215535⟩ PLoS ONE, Vol 14, Iss 4, p e0215535 (2019) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0215535⟩ |
| Popis: | International audience; β-(1→3)-D-Glucan is an essential component of the fungal cell wall. Mouse monoclonal antibodies (mAbs) against synthetic nona-β-(1→3)-D-glucoside conjugated with bovine serum albumin (BSA) were generated using hybridoma technology. The affinity constants of two selected mAbs, 3G11 and 5H5, measured by a surface plasmon resonance biosensor assay using biotinylated nona-β-(1→3)-D-glucan as the ligand, were approximately 11 nM and 1.9 nM, respectively. The glycoarray, which included a series of synthetic oligosaccharide derivatives representing β-glucans with different lengths of oligo-β-(1→3)-D-glucoside chains, demonstrated that linear tri-, penta- and nonaglucoside, as well as a β-(1→6)-branched octasaccharide, were recognized by mAb 5H5. By contrast, only linear oligo-β-(1→3)-D-glucoside chains that were not shorter than pentaglucosides (but not the branched octaglucoside) were ligands for mAb 3G11. Immunolabelling indicated that 3G11 and 5H5 interact with both yeasts and filamentous fungi, including species from Aspergillus, Candida, Penicillium genera and Saccharomyces cerevisiae, but not bacteria. Both mAbs could inhibit the germination of Aspergillus fumigatus conidia during the initial hours and demonstrated synergy with the antifungal fluconazole in killing C. albicans in vitro. In addition, mAbs 3G11 and 5H5 demonstrated protective activity in in vivo experiments, suggesting that these β-glucan-specific mAbs could be useful in combinatorial antifungal therapy. |
| Databáze: | OpenAIRE |
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