Bacillus iranensis sp. nov., a moderate halophile from a hypersaline lake
| Autor: | M. Mehrshad, M. A. Amoozegar, M. Didari, Cristina Sánchez-Porro, Antonio Ventosa, Maryam Bagheri, Peter Schumann |
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| Rok vydání: | 2012 |
| Předmět: |
DNA
Bacterial Geologic Sediments Salinity Molecular Sequence Data Bacillus Peptidoglycan Diamino acid Iran Sodium Chloride Diaminopimelic Acid Microbiology Endospore chemistry.chemical_compound RNA Ribosomal 16S Phospholipids Phylogeny Ecology Evolution Behavior and Systematics Spores Bacterial Base Composition biology Strain (chemistry) Fatty Acids Nucleic Acid Hybridization Vitamin K 2 Sequence Analysis DNA General Medicine Hypersaline lake biology.organism_classification 16S ribosomal RNA Halophile Bacterial Typing Techniques Lakes chemistry Water Microbiology Bacteria |
| Zdroj: | International Journal of Systematic and Evolutionary Microbiology. 62:811-816 |
| ISSN: | 1466-5034 1466-5026 |
| Popis: | A Gram-positive, moderately halophilic rod, designated X5BT, was isolated from saline mud of the hypersaline lake Aran-Bidgol in Iran. Strain X5BT was a strictly aerobic, motile bacterium that produced ellipsoidal endospores at a central-subterminal position in non-swollen sporangia. The isolate grew at pH 7.0–10.0 (optimum pH 7.5), at 25–45 °C (optimum 35 °C) and with 2.5–15 % (w/v) NaCl (optimum 5–7.5 %). On the basis of 16S rRNA gene sequences, strain X5BT belonged to the genus Bacillus and showed highest similarity with Bacillus persepolensis HS136T (95.6 % 16S rRNA gene sequence similarity) and Bacillus salarius BH169T (95.5 %). The DNA G+C content was 42.4 mol%. The major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0 and the polar lipid profile consisted of phosphatidylglycerol, diphosphatidylglycerol, three phospholipids and two glycolipids. The diamino acid found in the cell-wall peptidoglycan was meso-diaminopimelic acid and the isoprenoid quinones were MK-7 (92 %), MK-6 (6 %) and MK-5 (2 %). On the basis of phylogenetic, chemotaxonomic and phenotypic data, a novel species of the genus Bacillus is proposed, with the name Bacillus iranensis sp. nov. The type strain is X5BT ( = IBRC 10446T = DSM 23995T). |
| Databáze: | OpenAIRE |
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