Regulation of the Bacillus subtilis Heat Shock Gene htpG Is under Positive Control
| Autor: | Saskia Versteeg, Andy Wende, Angelika Escher, Wolfgang Schumann, Thomas Wiegert |
|---|---|
| Rok vydání: | 2003 |
| Předmět: |
Hot Temperature
HSPA12A Base Sequence biology Molecular Sequence Data Sigma Factor Gene Expression Regulation Bacterial Bacillus subtilis Regulatory Sequences Nucleic Acid biology.organism_classification Microbiology Molecular biology HSPA1B Bacterial Proteins Regulatory sequence Sigma factor Heat shock protein Gene Regulation HSP90 Heat-Shock Proteins Heat shock Molecular Biology Gene Heat-Shock Response |
| Zdroj: | Journal of Bacteriology. 185:466-474 |
| ISSN: | 1098-5530 0021-9193 |
| DOI: | 10.1128/jb.185.2.466-474.2003 |
| Popis: | The heat shock genes of Bacillus subtilis are assigned to four classes on the basis of their regulation mechanisms. While classes I and III are negatively controlled by two different transcriptional repressors, class II is regulated by the alternative sigma factor σ B . All heat shock genes with unidentified regulatory mechanisms, among them htpG , constitute class IV. Here, we show that expression of htpG is under positive control. We identified a DNA sequence (GAAAAGG) located downstream of the σ A -dependent promoter of htpG . The heat inducibility of the promoter could be destroyed by inversion, nucleotide replacements, or removal of this DNA sequence. Fusion of this sequence to the vegetative lepA promoter conferred heat inducibility. Furthermore, we were able to show that the heat induction factor is dependent on the absolute temperature rather than the temperature increment and that nonnative proteins within the cytoplasm fail to induce htpG . |
| Databáze: | OpenAIRE |
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