Electroporation of Single Cells and Tissues with an Electrolyte-filled Capillary
| Autor: | Cecilia Farre, Stephen G. Weber, Owe Orwar, Anke Brederlau, Kerstin Nolkrantz, Peter S. Eriksson, Mats Sandberg, Carrie Brennan, Roger Karlsson |
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| Rok vydání: | 2001 |
| Předmět: |
chemistry.chemical_classification
Patch-Clamp Techniques Chemistry Biomolecule Electroporation Inositol 1 4 5-Trisphosphate Electrolyte Hippocampus Molecular biology Cell Line Rats Analytical Chemistry Rats Sprague-Dawley Electrolytes Membrane Microscopy Fluorescence Cytoplasm Cell culture Culture Techniques Organelle Fluorescence microscope Biophysics Animals |
| Zdroj: | Analytical Chemistry. 73:4469-4477 |
| ISSN: | 1520-6882 0003-2700 |
| Popis: | We show how an electrolyte-filled capillary (EFC) coupled to a high-voltage power supply can be used as a versatile electroporation tool for the delivery of dyes, drugs, and biomolecules to the cytoplasm of single cells and cells in tissues. A large-voltage pulse applied across the EFC (fused silica, 30 cm long, 375-microm o.d., 30-microm i.d.) gives rise to a small electric field outside the terminus of the EFC, which causes pore formation in cell membranes and induces an electroosmotic flow of electrolyte. When the EFC contains cell-loading agents, then the electroosmotic flow delivers the agents at the site of pore formation. The combination of pore formation and delivery enables loading of materials into the cytoplasm. By patch-clamp and fluorescence microscopy, formation of pores was observed at estimated transmembrane voltages of |
| Databáze: | OpenAIRE |
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