Selective inhibition by alcohol and cortisol of natural killer cell activity of lymphocytes from cord blood
| Autor: | U.J. Dumaswala, Stanley A. Schwartz, Ann M. Sweet, Zaid A. Kronfol, Madhavan Nair, John F. Greden, Kailash C. Chadha |
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| Rok vydání: | 1994 |
| Předmět: |
Adult
Cytotoxicity Immunologic Male endocrine system medicine.medical_specialty Cellular immunity Hydrocortisone Lymphocyte Alcohol and cortisol Biology In Vitro Techniques Natural killer cell Cell Line Internal medicine medicine Humans Lymphocytes Cytotoxicity Biological Psychiatry Pharmacology Ethanol Infant Newborn Fetal Blood Chromium Radioisotopes Killer Cells Natural medicine.anatomical_structure Endocrinology Cord blood Toxicity Female Leukemia Erythroblastic Acute medicine.drug |
| Zdroj: | Progress in neuro-psychopharmacologybiological psychiatry. 18(8) |
| ISSN: | 0278-5846 |
| Popis: | 1. 1. The immunosuppressive effects of drugs such as alcohol or hormones such as cortisol may be age-related. To test this hypothesis, the authors investigated the in vitro effects of ethanol (EtOH) and cortisol on Natural Killer (NK) cell activity of lymphocytes from normal cord blood in comparison with that of lymphocytes from normal adult peripheral blood. 2. 2. K562, an erythroleukemia cell line, was used as a target in a 4 hr 51 Cr release assay. 3. 3. Ethanol at 0.3% (V/V) and cortisol at 0.05, 0.1 and 0.2 μg/ml concentrations, added directly to a mixture of effector and target cells significantly suppressed the NK activity of cord blood lymphocytes in a dose dependent fashion, whereas similar concentrations of either EtOH or cortisol did not manifest significant immunoregulatory effects on NK cell activity of normal adult lymphocytes. 4. 4. Pre-treatment of the target with either EtOH or cortisol for 4 hours did not affect cytotoxicity. Inhibition of cytotoxicity was also not due to direct toxicity of effector cells because lymphocytes treated with either EtOH or cortisol showed normal 51 Cr release and their viability was comparable to that of untreated control cells. 5. 5. This suggests a selective inhibitory effect of EtOH and cortisol on NK activity of neonatal lymphocytes that may be of clinical significance. |
| Databáze: | OpenAIRE |
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