Immunohistochemical analyses point to epidermal origin of human Merkel cells
| Autor: | Pia Houdek, Thomas Tilling, Antonio Virgilio Failla, Ingrid Moll, E Wladykowski, Johanna M. Brandner |
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| Rok vydání: | 2013 |
| Předmět: |
Pathology
medicine.medical_specialty animal structures Histology Cellular differentiation Keratin-20 Biology Merkel Cells Neurosphere medicine Humans Paired Box Transcription Factors Cell Lineage Progenitor cell Molecular Biology PAX3 Transcription Factor Membrane Glycoproteins Microscopy Confocal integumentary system SOXE Transcription Factors Keratin 20 Integrin beta1 Neural crest Cell Biology Immunohistochemistry Neural stem cell Cell biology Medical Laboratory Technology medicine.anatomical_structure Epidermal Cells Stem cell Epidermis Merkel cell |
| Zdroj: | Histochemistry and cell biology. 141(4) |
| ISSN: | 1432-119X |
| Popis: | Merkel cells, the neurosecretory cells of skin, are essential for light-touch responses and may probably fulfill additional functions. Whether these cells derive from an epidermal or a neural lineage has been a matter of dispute for a long time. In mice, recent studies have clearly demonstrated an epidermal origin of Merkel cells. Given the differences in Merkel cell distribution between human and murine skin, it is, however, unclear whether the same holds true for human Merkel cells. We therefore attempted to gain insight into the human Merkel cell lineage by co-immunodetection of the Merkel cell marker protein cytokeratin 20 (CK20) with various proteins known to be expressed either in epidermal or in neural stem cells of the skin. Neither Sox10 nor Pax3, both established markers of the neural crest lineage, exhibited any cell co-labeling with CK20. By contrast, β1 integrin, known to be enriched in epidermal stem cells, was found in nearly 70 % of interfollicular epidermal and 25 % of follicular Merkel cells. Moreover, LRIG1, also enriched in epidermal stem cells, displayed significant co-immunolabeling with CK20 as well (approximately 20 % in the interfollicular epidermis and 7 % in the hair follicle, respectively). Further epidermal markers were detected in sporadic Merkel cells. Cells co-expressing CK20 with epidermal markers may represent a transitory state between stem cells and differentiated cells. β1 integrin is probably also synthesized by a large subset of mature Merkel cells. Summarizing, our data suggest that human Merkel cells may originate from epidermal rather than neural progenitors. |
| Databáze: | OpenAIRE |
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