Expression of matrix metalloproteinases-2 and -9 in intestinal tissue of patients with inflammatory bowel diseases
| Autor: | Hein W. Verspaget, R. A. van Hogezand, Cornelis B.H.W. Lamers, L. Kruidenier, M. van den Berg, W. van Duijn, Cornelis F. M. Sier, M.J.W. Meijer, F.J.G.M. Kubben, Qiang Gao |
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| Rok vydání: | 2005 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Neutrophils Enzyme-Linked Immunosorbent Assay Matrix metalloproteinase Matrix (biology) Severity of Illness Index Inflammatory bowel disease Basement Membrane Extracellular matrix Submucosa Humans Medicine RNA Messenger Aged DNA Primers Basement membrane Crohn's disease Hepatology Reverse Transcriptase Polymerase Chain Reaction business.industry Gastroenterology Epithelial Cells Fibroblasts Middle Aged Inflammatory Bowel Diseases medicine.disease Immunohistochemistry Ulcerative colitis Intestines medicine.anatomical_structure Matrix Metalloproteinase 9 Case-Control Studies Matrix Metalloproteinase 2 Female business |
| Zdroj: | Digestive and Liver Disease. 37:584-592 |
| ISSN: | 1590-8658 |
| DOI: | 10.1016/j.dld.2005.02.011 |
| Popis: | Background/aims. Matrix metalloproteinases are major contributors in the breakdown and reconstitution of basement membranes and extracellular matrix in pathophysiological processes. We assessed the expression of matrix metalloproteinases-2 and -9 in intestinal tissue of patients with inflammatory bowel disease. Patients/methods. Resected tissue specimens from patients with Crohn's disease or ulcerative colitis and control tissue from patients with a colorectal carcinoma were used for enzyme-linked immunosorbent assay, zymography, activity assay, reverse transcription polymerase chain reaction and immunohistochemistry to evaluate the expression of these matrix metalloproteinases. Results. Matrix metalloproteinase-2 and more strongly matrix metalloproteinase-9 protein and mRNA were markedly increased in inflammatory bowel disease tissues, with the highest levels in severely inflamed tissues. Immunohistochemistry showed that matrix metalloproteinase-2 was present in the extracellular matrix of the submucosa, with a lower but more generalised expression in the severely inflamed regions. Matrix metalloproteinase-9 was most prominent in polymorphonuclear leukocytes and was increased, also in activity, in all inflammatory bowel disease tissues. An increased matrix metalloproteinase-9 expression in the extracellular matrix was observed in relation to the severity of inflammation. Conclusions. Matrix metalloproteinases-2 and -9 are enhanced in the intestinal tissue and seem to be actively involved in the inflammatory and remodelling processes in inflammatory bowel disease, without major differences between CD and UC. |
| Databáze: | OpenAIRE |
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