Proximity labeling: an emerging tool for probing in planta molecular interactions
| Autor: | Zhen Li, Yongliang Zhang, Savithramma P. Dinesh-Kumar, Xinxin Yang, Dingliang Zhang, Zhiyan Wen, Dawei Li, Ugrappa Nagalakshmi |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Cellular activity
Quantitative proteomics membrane contact sites plant Plant Science Computational biology Review Article Biology Biochemistry protein interactions Protein–protein interaction Protein Interaction Maps Plant system Molecular Biology Organism Plant Proteins Molecular interactions Staining and Labeling Botany Cell Biology Plant biology organelles Identification (biology) biotin ligase Biotechnology proximity labeling |
| Zdroj: | Plant Communications |
| ISSN: | 2590-3462 |
| Popis: | Protein–protein interaction (PPI) networks are key to nearly all aspects of cellular activity. Therefore, the identification of PPIs is important for understanding a specific biological process in an organism. Compared with conventional methods for probing PPIs, the recently described proximity labeling (PL) approach combined with mass spectrometry (MS)-based quantitative proteomics has emerged as a powerful approach for characterizing PPIs. However, the application of PL in planta remains in its infancy. Here, we summarize recent progress in PL and its potential utilization in plant biology. We specifically summarize advances in PL, including the development and comparison of different PL enzymes and the application of PL for deciphering various molecular interactions in different organisms with an emphasis on plant systems. Proximity labeling (PL) has emerged as a powerful approach for the characterization of protein–protein interactions. This review summarizes advances in PL, including the development and comparison of different PL enzymes and the application of PL for dissecting molecular interactions in different organisms with an emphasis on plant systems. |
| Databáze: | OpenAIRE |
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