HIP/PAP Gene, Encoding a C-Type Lectin Overexpressed in Primary Liver Cancer, Is Expressed in Nervous System as well as in Intestine and Pancreas of the Postimplantation Mouse Embryo
| Autor: | Christian Bréchot, Céline Colnot, Chantal Lasserre, Françoise Poirier |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Nervous system
Pathology medicine.medical_specialty Carcinoma Hepatocellular Embryonic Development Pancreatitis-Associated Proteins Enteroendocrine cell In situ hybridization Biology Pathology and Forensic Medicine Andrology Embryonic and Fetal Development Mice Pregnancy Lectins Gene expression medicine Animals Humans Northern blot Intestinal Mucosa Pancreas In Situ Hybridization Reverse Transcriptase Polymerase Chain Reaction Liver Neoplasms Gene Expression Regulation Developmental Embryo Small intestine Gene Expression Regulation Neoplastic medicine.anatomical_structure Genetic Code Female Regular Articles |
| Zdroj: | The American Journal of Pathology. 154:1601-1610 |
| ISSN: | 0002-9440 |
| DOI: | 10.1016/s0002-9440(10)65413-2 |
| Popis: | We originally isolated the HIP/PAP gene in a differential screen of a human hepatocellular carcinoma cDNA library. This gene is expressed at high levels in 25% of primary liver cancers but not in nontumorous liver. HIP/PAP belongs to the family of C-type lectins and acts as an adhesion molecule for hepatocytes. In normal adult human tissues, HIP/PAP expression is found in pancreas (exocrine and endocrine cells) and small intestine (Paneth and neuroendocrine cells). In order to gain insight into the possible role of HIP/PAP in vivo, we have investigated the pattern of HIP/PAP expression in the developing postimplantation mouse embryo by in situ hybridization. Detailed analysis of developing mouse embryos revealed that HIP/PAP gene exhibits a restricted expression pattern during development. Thus, HIP/PAP transcripts are first observed within the nervous system from day 14.5 onwards in trigeminal ganglia, dorsal root ganglia, and spinal cord where it appears to be an early specific marker of a subpopulation of motor neurons. At laster stages, HIP/PAP transcripts were detected in intestine and pancreas at day 16.5 but not in embryonic liver. This highly restricted expression pattern suggests that HIP/PAP might participate in neuronal as well as intestinal and pancreatic cell development. |
| Databáze: | OpenAIRE |
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